The pulldown assay was performed as previously described [14 (link)]. Briefly, to generate probe-coated beads, the biotinylated circUSP7 probe, biotinylated circANRIL probe, and biotinylated NC probe (GenePharma, China) were incubated with M-280 streptavidin magnetic beads (Invitrogen, USA, Cat: 11205D) at room temperature for 2 h. Then, approximately 1 × 107 CD8+ T cells were harvested, lysed, and sonicated, and these lysates were incubated with the probe-coated beads at 4 °C overnight. Subsequently, the RNA complexes bound to the beads were eluted and extracted with an RNeasy Mini Kit (QIAGEN, USA, Cat: 74104) and analysed by qRT-PCR.
Biotinylated nc probe
Manufactured by GenePharma
Sourced in China
The Biotinylated NC probe is a laboratory tool used for nucleic acid detection and analysis. It consists of a DNA or RNA sequence that is labeled with biotin, a small molecule that can bind to streptavidin or avidin. This probe can be used to identify and locate specific genetic sequences in a sample, such as during Southern blotting or Northern blotting procedures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using biotinylated nc probe
1
CircRNA pulldown assay for CD8+ T cells
2
Biotinylated Probes for NCAPH Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pull‐down assay was carried out as previously described.33 The biotinylated NCAPH probe, biotinylated pre‐NCAPH probe and biotinylated NC probe were produced by GenePharma. The precipitated proteins were identified by Western blotting.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!