Dulbecco’s modified Eagle’s medium (DMEM), Ham’s F-12 nutrient mixture, fetal bovine serum (FBS), L-glutamine, penicillin and streptomycin, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl formazan (MTT), α,α-Diphenyl-β-picrylhydrazyl (DPPH), 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt (Ferrozine™), Thioflavin T (ThT), Folin–Ciocalteau’s reagent, hen egg white lysozyme (HEWL), gallic acid, ascorbic acid, D-glucose and all other chemicals, analytical grade and HPLC grade solvents were purchased from Sigma Aldrich-Merck (Saint Louis, MO, USA). Disposable plastics were from Sarstedt (Nümbrecht, Germany).
1 4 5 dimethylthiazol 2 yl 3 5 diphenylformazan
Manufactured by Merck Group
Sourced in United States
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan is a chemical compound used in laboratory settings. It is a tetrazolium salt that is commonly used as a colorimetric indicator in cell viability assays.
Automatically generated - may contain errors
Lab products found in correlation
Ascorbic acid, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
D glucose, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Thiazolyl blue formazan mtt, by Merck Group (2 mentions)
Disposable plastics, by Sarstedt (1 mentions)
Ham s f 12 nutrient mixture, by Merck Group (1 mentions)
Folin ciocalteau s reagent, by Merck Group (1 mentions)
Hen egg white lysozyme, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Thioflavin t, by Merck Group (1 mentions)
Histopaque 1077, by Merck Group (1 mentions)
Crystal violet powder, by Merck Group (1 mentions)
Ab16491, by Abcam (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Eif4e, by Cell Signaling Technology (1 mentions)
Magnesium chloride, by Merck Group (1 mentions)
Osi 906, by MedChemExpress (1 mentions)
8 protocols using 1 4 5 dimethylthiazol 2 yl 3 5 diphenylformazan
1
Comprehensive Cell Culture Protocol
2
Evaluation of M. oleifera and P. frutescens Seed Oils
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Cold-pressed M. oleifera seed oil originating from India was purchased from Neo Moringa (Bangkok, Thailand). P. frutescens seed oil was obtained from Pang Oung local market (Meahongsorn, Thailand). Caprylic triglyceride (Lexol®865), polysorbate 20 (Tween 20) polysorbate 80 (Tween 80) and propylene glycol were purchased from Namsiang (Bangkok, Thailand). Roswell Park Memorial Institute 1640 medium (RPMI-1640), 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan (MTT reagent), and histopaque-1077 were purchased from Sigma-Aldrich, St. Louis, MO, USA). Xanthan gum, glycerin, PEG/PPG-14/7 dimethyl ether, phenoxyethanol, and tocopheryl acetate were obtained from Myskinrecipe (Bangkok, Thailand).
3
Reagents and Antibodies for Cell Culture
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Cell culture reagents were purchased from Trace Biosciences (North Ryde, New South Wales, Australia) and Nunc (Roskilde, Denmark). Bovine insulin, methanol, calcium chloride, magnesium chloride, crystal violet powder and 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan were purchased from Sigma-Aldrich. Enhanced chemiluminescence (ECL) reagent was SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology). The dual IGF1R/insulin receptor tyrosine kinase inhibitor (OSI-906; also referred to as linsitinib) was purchased from MedChem Express (Princeton, NJ) and the SphK inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SKI-II) from Calbiochem [21 (link)]. Antibodies raised against phospho-Y1135/1136 IGFR1, IGFR1 beta chain, phospho-Ser473 AKT and total AKT, 4E-BP1 and eIF4E were purchased from Cell Signaling Technology (Beverley, MA). The antibody to detect SphK1 (ab16491) for western blots was purchased from Abcam and for SphK1 immunohistochemistry, from Abgent (AP7237c).
4
Eudesmin Cytotoxicity Evaluation Protocol
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Eudesmin (purity >98%) was obtained from Chengdu Herbperity Co., Ltd (Chengdu, China). 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-formazan (MTT) was purchased from Sigma-Aldrich (St. Louis, MO). The primary monoclonal antibodies of P53, Bcl-2, Bax, caspase-9, caspase-3, Akt, JNK and horseradish peroxidase-conjugated secondary antibodies were obtained from Beyotime Biotechnology (Shanghai, China). RPMI1640 culture medium and foetal bovine serum (FBS) were purchased from American Hyclone Company (Logan, UT). All other chemicals and reagents used in this study were of analytical grade.
5
Liposomal Doxorubicin Formulation and Characterization
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1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC), (N-(carbonyl-methoxypolyethylene glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine, and sodium salt) (DSPE-mPEG2000), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were purchased from Lipoid AG (Steinhausen, Switzerland). 1-Stearoyl-2-lyso-sn-glycero-3-phosphocholine (MSPC, S-LysoPC) was purchased from NOF America Corporation (White Plains, NY, USA). Doxorubicin hydrochloride (DOX) was purchased from Gemini Pharmaceuticals Inc., (Hauppauge, NY, USA). Cholesterol, ammonium sulfate, hydrochloric acid, sodium hydroxide, 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, thiazolyl blue formazan (MTT), and L-histidine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sucrose was purchased from CheilJedang (Seoul, Korea). An Oasis HLB 3 cc Vac Cartridge (solid-phase extraction column; SPE column) was purchased from Waters (Milford, MA, USA).
6
Antioxidant and Cytotoxicity Assays
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3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4″-disulfonic acid sodium salt (Ferrozine®), α,α-diphenyl-β-picrylhydrazyl (DPPH), Folin–Ciocalteau’s phenol reagent, gallic acid, ascorbic acid, d -glucose, gelatin, Coomassie Brilliant Blue R-250, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT), Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS) and Bovine Serum Albumin (BSA) were all purchased from Sigma Aldrich-Merck (St. Louis, MO, USA). 30% Acrylamide/Bis 37.5:1 solution, ammonium persulfate (APS), 1,2-Bis (dimethylamino) ethane (TEMED), Tris/Glycine buffers, nitrocellulose membranes (0.45 μM) blotting membranes and Clarity Western ECL solution were purchased from Bio-Rad. Disposable plastics were from Corning. Photometric measurements from multi-well plates were recorded on an iMARK microplate reader (Bio-Rad, Hercules, CA, USA). When not otherwise specified, all chemicals and solvents, such as ethanol, methanol and n-hexane, were of the highest analytical grade and were purchased from Sigma Aldrich-Merck.
7
MTT Cytotoxicity Assay Protocol
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1-(4, 5-Dimethylthiazol-2-yl)-3, 5-diphenylformazan or thiazolyl blue formazan (MTT), finasteride and hydrocortisone were purchased from Sigma-Aldrich (St. Louis, USA). Fetal bovine, 2.5% trypsin serum, penicillin/streptomycin, phosphate buffer solution pH 7.4 were obtained from Gibco (Auckland, New Zealand). Dimethyl sulfoxide or DMSO (Analytical grade) was purchased from Labscan (V.S. Chem House, Thailand). Mouse IL-1β ELISA Instant Kit was purchased from e-bio sciences (Bender-med systems Gmblt, Vienna, Austria). Follicle dermal papilla cell growth medium was purchased from PromoCell (Heidelberg, Germany).
8
Microbial Viability Evaluation Methods
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Yeast extract Peptone Dextrose medium (YPD), Luria–Bertani (LB) and agar were purchased from BTL (Poland). Acridine orange (AO), 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT), crystal violet (CV), ethanol were purchased from Sigma-Aldrich (USA). Dimethyl sulfoxide (DMSO) and ethidium bromide (EtBr) were purchased from Chempur and MpBio, respectively. Dulbecco's Modified Eagle Medium High Glucose (DMEM, Corning), antibiotic solution (Antibiotic Antimycotic Solution, Sigma-Aldrich), fetal bovine serum (FBS, Biowest), phosphate buffered saline w/o magnesium and calcium (PBS, Corning), 0.25% trypsin (PAA) were used.
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