Tissue microarray (Auragene, Changsha, China) containing 80 cervical cancer specimens was prepared for immunochemistry. Antigen retrieval was performed in pH 6.0 citrate buffers, by using a pressure cooker at 104°C for 32 min with a 10 min bench cool down, followed by quenching with 3% H2O2 w/sodium azide for 15 min. After blocking in a serum-free protein block for 1 h, P65 antibody (1:100, Immunoway, Newark, USA), phospho-P65 antibody (1:100), PI3K antibody (1:100), phospho-PI3K antibody (1:100), STC1 antibody (1:100, santa cruz, Texas, USA) was incubated with the samples for overnight at 4°C, then the tissue microarray was detected with Dako Envision + HRP Labeled Polymer (Auragene, Changsha, China) for 30 min after incubating with chromogen DAB+ for 30 s and hematoxylin for 15 min. The sum of IOD of STC1 expression was determined by IPP6.0.
Pi3k antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The PI3K antibody is a laboratory reagent used to detect and study the phosphoinositide 3-kinase (PI3K) enzyme. PI3K is a key signaling molecule involved in various cellular processes, including cell growth, proliferation, and metabolism. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to identify and quantify the presence of PI3K in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Phospho erk antibody, by Cell Signaling Technology (2 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Mtor antibody, by Abcam (1 mentions)
Fusion fx5 spectra, by Vilber (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
Hrp conjugated secondary antibody, by ZSGB-BIO (1 mentions)
Akt antibody, by Cell Signaling Technology (1 mentions)
4 protocols using pi3k antibody
1
Immunochemistry Profiling of Cervical Cancer
2
Western Blot Analysis of AKT/PI3K/mTOR Pathway
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Whole cell lysates were separated with 10% SDS-PAGE and transferred to nitrocellulose membrane (Millipore, MA). Following being blocked with 5% skimmed milk to block non-specific proteins for 1 h at room temperature, membranes were treated with corresponding primary antibody overnight at 4°C, and then followed by peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies for 1 h at room temperature. Protein bands were visualized using the Tanon High-sig ECL (Tanon Science and Technology Co., Ltd., Shanghai, China) and analyzed with Vilber Fusion Fx5 Spectra (Vilber Lourmat, Marne La Vallée, France). The anti-DEPDC1 antibody was purchased from abcam Inc. (Abcam, Cambridge, MA, USA). GAPDH antibody was obtained from Santa Inc (Dallas, TX, USA). AKT antibody (Cell Signaling Technology, USA), p-AKT (Ser473) (Cell Signaling Technology, USA), PI3K antibody (Santa Cruz, USA), p-PI3K p85 (Tyr458) antibody (Santa Cruz, USA), mTOR antibody (Abcam, USA) and p-mTOR (Ser2448) antibody (Cell Signaling Technology, USA) were used to analyze AKT/PI3K/mTOR signal pathway. HRP-conjugated secondary antibodies were from ZSGB-BIO (Beijing, China).
3
Peptide Effects on Hair Follicle Cells
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Example 3
Human hair follicle dermal papilla cells were seeded at a density of 4×105 cells/well on 6-well plates, followed by incubation overnight. After changing into serum-free medium, the cells were treated with the peptides, followed by incubation for 15 and 30 minutes, and then the wells were harvested to prepare the cell lysate. Western blotting was performed using PI3K antibodies (Santa Cruz Biotechnology, USA) and phospho-ERK antibody (Cell Signaling Technology, USA) to compare protein expression patterns. The results are shown in
As can be confirmed from
4
Effect of Peptides on Hair Follicle Cells
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Example 4
Human hair follicle dermal papilla cells were seeded at a density of 4×105 cells/well on 6-well plate, followed by incubation overnight. After changing into serum-free medium, the cells were treated with the peptides, followed by incubation for 15 and 30 minutes, and then the wells were harvested to prepare cell lysate. Western blotting was performed using PI3K antibodies (Santa Cruz Biotechnology, USA) and phospho-ERK antibody (Cell Signaling Technology, USA) to compare protein expression patterns. The results are shown in
As can be confirmed from
In addition, as can be confirmed n
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