The indicators required to determine the ANI include AST, ALT, MCV, gender, height, and weight. Clinical parameters of height, weight, waist circumference, and blood pressure were recorded on the admission day, while serum samples were collected during the following morning after fasting for 12 hours. The biochemical indicators, including AST, ALT, MCV, GGT, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and fasting blood glucose (FBG), were detected by the Hitachi 7180 automatic biochemical analyzer (Hitachi, Tokyo, Japan) and supporting reagents. The ANI value was calculated with an online calculator provided by the Mayo Clinic (Rochester, MN, USA, official website: http://www.mayoclinic.org/gi-rst/mayomodel10.html ).
7180 automatic biochemical analyzer
Manufactured by Hitachi
Sourced in Japan, China
The Hitachi 7180 automatic biochemical analyzer is a compact and reliable instrument designed for clinical laboratory testing. It is capable of performing a wide range of biochemical analyses, including measurements of enzymes, substrates, and other clinical chemistry parameters. The instrument features automated sample handling, reagent dispensing, and data processing capabilities, allowing for efficient and accurate analysis of patient samples.
Automatically generated - may contain errors
Lab products found in correlation
Elisa kit, by Shanghai Enzyme-linked (3 mentions)
Synchron dxc800 automatic biochemical analyzer, by Beckman Coulter (1 mentions)
Electrochemical luminescence, by Roche (1 mentions)
Fibroscan 502, by Echosens (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Glutathione peroxidase gsh px, by Nanjing Jiancheng (1 mentions)
Nit 1, by Procell (1 mentions)
Accu check blood glucose meter, by Roche (1 mentions)
D 10 analyzer, by Bio-Rad (1 mentions)
Autoanalyzer, by Roche (1 mentions)
Sdldl seiken kit, by Denka Seiken (1 mentions)
Xn 2000 hematology analyzer, by Sysmex (1 mentions)
Vacutainer serum collection tube, by BD (1 mentions)
Detection kit, by Nanjing Jiancheng (1 mentions)
Bc 2800vet, by Mindray (1 mentions)
Cobas e602, by Roche (1 mentions)
44 protocols using 7180 automatic biochemical analyzer
1
Assessing Nonalcoholic Liver Index Determinants
2
Urinary Protein and Kidney Function Assays
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Urinary total protein (Pyrogallol red-molybdate method) was measured by 7180 automatic biochemical analyzer (Hitachi). For proteinuria, there was 1 missing value in patient with negative serum anti-PLA2R antibody and positive glomerular PLA2R deposition. Serum total protein (Biuret method), serum albumin (Bromcresol green method), serum creatinine (Jaffe method), and serum urea (Urease methods) were measured by Synchron DXC800 automatic biochemical analyzer (Beckman Coulter). Estimated glomerular filtration rate (eGFR) levels were calculated using the Chronic Kidney Disease Epidemiology Collaboration equation recommended by the Kidney Disease Improving Global Outcomes.[9 (link)]
3
Comprehensive Metabolic Profiling of Fasting Patients
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The venous blood was collected from the peripheral forearm of the enrolled patients after an overnight fast of 8–12 hours. TC, TG, HDL, LDL, liver, kidney function tests, and other biochemical indices were measured using the Hitachi 7180 automatic biochemical analyzer. HBA1c was determined by high-pressure liquid chromatography. An OGTT, insulin release test, and glucagon release test were performed. Patients were inhibited from using insulin secretagogue three day before the blood draw. 75 g glucose was administered orally after an overnight fast of 12 hours. Venous blood from the forearm was collected at 0, 30, 60, 120, and 180 mins, respectively, to evaluate the FBG and 2hPBG, insulin and glucagon levels. The glucose oxidase method was used to assess glucose levels. Insulin was measured by electrochemical luminescence (Roche, Germany), and glucagon was measured by nonequilibrium radioimmunity.
4
Serum Biochemical Analysis in Mice
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Serum was collected after the mice were sacrificed and was centrifugated at 3000 × g for 10 min. Then, the serum was subjected to liver and kidney function analysis using the Hitachi 7180 automatic biochemical analyzer (Tokyo, Japan). The rate method was used to measure aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH); the hexokinase method was used to measure glucose (GLU); the enzymatic method was used to measure creatinine (Cr) and UREA; the uricase ultraviolet method was used to measure uric acid (UA); the bromocresol green method was used to measure albumin (ALB); the biuret method was used to measure total protein (TP) and globulin (GLOB).
5
Comparison of 14 Routine Biochemical Assays
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Among the 14 routine methods, three were from foreign companies (Roche, Diasys, and Wako), and the others were from Chinese companies (Sichuan Maccura, Beijing Strong, DIRUI, Leadman, Mindray, Shanghai Fosun Long March, Kehua, Reebio, Biosino, Long March I (OCPC), and Long March II [the azo arsenic III method]). All tests were completed on a Hitachi 7180 automatic biochemical analyzer and the parameters were set in accordance with the manufacturer's instructions. Calibration was performed with supporting calibrators before the measurements. All of the samples were detected twice, and their order of measurement was reversed in the replicate run.
6
Biochemical Analysis of Hepatitis B
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A Hitachi 7180 automatic biochemical analyzer (Japan) performed routine biochemical tests during therapy at the follow-up point. Serum HBsAg levels, antibody to HBsAg (HBsAb), HBeAg, and antibody to HBeAg (HBeAb) were determined by an Architect I2000SR electrochemistry luminescence immunity analyzer (U.S.A). HBsAg and HBsAb levels were expressed in IU/mL and mIU/mL and had dynamic detection ranges which are 0–124,950 IU/mL and 0–1000 mIU/mL, respectively. HBeAg and HBeAb were determined semi-quantitatively and expressed as the signal-to-cut-off ratio (S/CO). Liver stiffness measurement (LSM) was assessed by FibroScan-502 (Echosens, Paris, France), equipped with an M-type probe at 3.5 MHz. The result for LSM was presented in kPa.
7
Evaluating Islet β Cell Dysfunction
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C57BL/6J mice (males and females, 5 weeks old, 16–18 g) were purchased from Guangzhou Cypress Biotech Co. The mouse islet β cell line NIT-1 was purchased from Wuhan Procell. DMEM medium, FBS, penicillin-streptomycin, and trypsin were purchased from Gibco (USA). Streptozocin (STZ)-citrate buffer was purchased from Sigma. Lipid peroxidation product malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were purchased from Nanjing Jiancheng Bioengineering Institute. The 7180 automatic biochemical analyzer was purchased from Hitachi (Japan). Gallios flow cytometry equipment was purchased from Beckman Coulter (USA). Annexin V-FITC/PI apoptosis detection kits were purchased from Beyotime (Jiangsu, China). Mice were used for all experiments, and all procedures were approved by the Animal Ethics Committee of our hospital.
8
Liver Function and Toxicity Assessment
9
Metabolic Profiling in Rat Model
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Rats were randomly grouped. Body weight and food intake were monitored daily during the experimental period. Fasting blood glucose level was monitored from vein every week using an Accu-check blood glucose meter (Roche Diagnostics, Basel, Switzerland). The serum free fatty acid (FFA), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C) and triglyceride (TG) levels were determined using a 7180-automatic biochemical analyzer (Hitachi, Japan) at the Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University (Xi'an, China).
10
Serum Biomarker Analysis Protocol
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The blood samples were centrifuged at 3500 rpm for 10 min at 4°C to separate the serum. The serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose and high-sensitivity C-reactive protein (hsCRP) contents were measured with a 7180 automatic biochemical analyzer (Hitachi Ltd., Tokyo, Japan).
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