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Brightvision horseradish peroxidase conjugated goat anti rabbit polyclonal secondary antibody

Manufactured by Immunologic

BrightVision horseradish peroxidase-conjugated goat anti-rabbit polyclonal secondary antibody is a laboratory reagent used to detect and visualize target proteins in various immunoassays. The antibody is conjugated with horseradish peroxidase, an enzyme that catalyzes a color-producing reaction, allowing for the detection of the target protein.

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2 protocols using brightvision horseradish peroxidase conjugated goat anti rabbit polyclonal secondary antibody

1

Immunohistochemical Analysis of NPM, p-NPM, Ki-67, and Cleaved Caspase-3

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The following antibodies were used: rabbit anti-human NPM polyclonal antibody (1:1000, Cell Signaling Technology); rabbit anti-human p-NPM (Thr199) polyclonal antibody (1:400, Cell Signaling Technology); rabbit anti-human Ki-67 (clone SP6) monoclonal antibody (1:1000, Thermo Scientific), rabbit anti-human cleaved caspase 3 (Asp175) polyclonal antibody (1:100, Cell Signaling Technology) and BrightVision horseradish peroxidase-conjugated goat anti-rabbit polyclonal secondary antibody (undiluted; 30 min; Immunologic). See Supplementary Materials and Methods for a detailed protocol.
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2

Immunohistochemical Analysis of Proliferation and Apoptosis

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The following antibodies were used: rabbit anti-human Ki-67 (clone SP6) monoclonal antibody (1:1,000, Thermo Scientific), rabbit anti-human cleaved caspase 3 (Asp175) polyclonal antibody (1:100, Cell Signaling Technology) and BrightVision horseradish peroxidase-conjugated goat anti-rabbit polyclonal secondary antibody (undiluted; 30 min; Immunologic). See Supplementary Materials and Methods for a detailed protocol.
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