Brains were harvested and left hemisphere fixed in Carnoy fixative (Giaccone et al., 2000 (link)), dehydrated and embedded in paraplast. Ten μm serial sections were stained with haematoxylin-eosin (H&E), or immunostained with monoclonal antibodies to PrP (6H4, 1:1000; Prionics) and to reactive astrocytes (GFAP, 1:2000; Abcam). Before PrP immunostaining, the sections were treated with proteinase K (10μg/mL, 5min, room temperature) and guanidine isothiocyanate (3M, 20min, room temperature). To prevent unspecific bindings, Animal Research Kit (ARK, Dako) was used. Immunoreactions were visualized using 3-3′-diaminobenzidine (DAB, Dako) as chromogen.
Animal research kit ark
Manufactured by Agilent Technologies
Sourced in Canada
The Animal Research Kit (ARK) is a comprehensive set of tools designed to support various animal research applications. The kit includes a collection of high-quality instruments and accessories essential for conducting a wide range of animal-based studies. The core function of the ARK is to provide researchers with the necessary equipment to safely and effectively handle, monitor, and analyze animal subjects during the research process.
Automatically generated - may contain errors
2 protocols using animal research kit ark
1
Histological Analysis of Prion Protein
2
Immunohistochemistry Protocol for Ki-67 and MF20
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The McMaster Centre for Gene Therapeutics core histology lab performed all immunohistochemistry. For Ki-67 and MF20, antigen retrieval was performed using heat-induced antigen retrieval in sodium citrate buffer (pH 6.0). For the detection of mouse and rabbit antibodies in mouse tissues, the Animal Research Kit (ARK, K3594, Dako Canada) and the Envision+ System-HRP labeled Polymer Anti-rabbit (K4003, Dako Canada) were used, respectively. The following primary antibody concentrations were used: Ki-67, 1:500; MF20, 1:100.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!