Adi spa 880

Manufactured by Enzo Life Sciences

Sourced in United States

The ADI-SPA-880 is a lab equipment product designed for laboratory use. It provides a core function of automated liquid handling, enabling precise and efficient liquid transfer capabilities.

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Lab products found in correlation

West zol plus western blot detection system, by iNtRON Biotechnology (2 mentions) Ab 2935893, by Enzo Life Sciences (1 mentions) G7781, by Merck Group (1 mentions) Ctb lc3 2 ic, by Cosmo Bio (1 mentions) F1804, by Merck Group (1 mentions) Ab1218, by Abcam (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) P0260, by Agilent Technologies (1 mentions) Alexa555 conjugated anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Ab8245, by Abcam (1 mentions) Ifn γ, by R&D Systems (1 mentions) Sc56766, by Santa Cruz Biotechnology (1 mentions) Anti flag antibody, by Merck Group (1 mentions) Anti his6 antibody, by Santa Cruz Biotechnology (1 mentions) Anti mouse igg conjugated with peroxidase, by Santa Cruz Biotechnology (1 mentions)

4 protocols using adi spa 880

Immunoblotting and Immunofluorescence Protocols

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Primary antibodies for immunoblotting: mouse anti-Ub (FK2; BML-PW8810 and UBCJ2; AB_2935893, Enzo Life Science), mouse anti-DnaK (8E2/2, ADI-SPA- 880, Enzo Life Science), goat anti-GroEL (ABIN6292975, antibodies-online), mouse anti-tubulin (E7, DSHB), rabbit anti-GST (G7781, Sigma) and rat anti-GFP (3H9, Proteintech).
Primary antibodies used for immunofluorescence microscopy: α-Ub (1:400, FK2; BML-PW8810 and UBCJ2; AB_2935893, Enzo Life Science) α-M1 (1:400, 1E3, ZRB2114, Merck) α-K63 (1:400, Apu3, 05–1308, Millipore) and α-LC3 (1:300, CTB-LC3–2-IC, Cosmo Bio,). Anti-Burkholderia antibody was provided by the United States Army Medical Research Institute of Infectious Diseases, an agency of the U.S. Government (“USAMRIID”).
Secondary antibodies used: Thermo Fisher Scientific (1:500, Alexa-conjugated anti-mouse, anti-goat and anti-rabbit antisera) and Dabco (1:5000, HRP-conjugated reagents).

Szczesna M., Huang Y., Lacoursiere R.E., Bonini F., Pol V., Koc F., Ward B., Geurink P.P., Pruneda J.N, & Thurston T.L. (2023). Dedicated bacterial esterases reverse lipopolysaccharide ubiquitylation to block immune sensing. Research Square.

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Western Blot Protein Analysis Protocol

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Whole-cell fractions (extracted by sonication or pellets) were dissolved in Laemmli’s sample buffer and boiled for 5 min. The protein samples were loaded onto a 12% SDS-polyacrylamide gel. Proteins were separated on the gel depending on the molecular weights and transferred to a polyvinylidene difluoride membrane. The membrane was blocked with 5% nonfat dry milk in 1× Tris-buffered saline-Tween 20 buffer and probed with anti-FLAG antibody (3:2000 dilution, F1804, Sigma, St. Louis, MO, USA) or anti-DnaK antibody (1:10,000 dilution, ADI-SPA-880, Enzo Life Science, Farmingdale, NY, USA) as primary antibodies. Anti-mouse IgG conjugated to peroxidase (3:5000 dilution, sc-2005, Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as the secondary antibody in all western blotting experiments. The chemiluminescent signals were developed with a West-Zol plus western blot detection system (Intron Biotechnology, Daejeon, Korea).

Kim H.J., Yoo W., Jin K.S., Ryu S, & Lee H.H. (2017). The role of the FliD C-terminal domain in pentamer formation and interaction with FliT. Scientific Reports, 7, 4418.

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Immunoblotting and Immunofluorescence Assay

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The following antibodies were used: rabbit anti-SEPT7 (1:1000 dilution, #18991, IBL), mouse anti-DnaK (1:5000, #ADI-SPA-880, Enzo), mouse anti-GFP (1:4000, #ab1218, abcam), rabbit anti-GBP1 (1:1000 #15303-1-AP, Proteintech), mouse anti-GAPDH (1:1000 or 1:2000, #ab8245, Abcam), goat HRP-conjugated anti-mouse (1:5000 or 1:10,000, #P0260, Dako), goat HRP-conjugated anti-rabbit (1:5000 or 1:10,000, #P0448, Dako), Alexa-555-conjugated anti-rabbit antibody (1:500, #10082602, ThermoFisher Scientific), Alexa-647-conjugated anti-rabbit antibody (1:500, #A27040, ThermoFisher Scientific). The following dyes and drug were used: Hoechst (1:300, #H3570, ThermoFisher Scientific), Alexa-488-conjugated phalloidin (1:300, #A12379, ThermoFisher Scientific), IFNγ (#285-IF, R&D Systems).

Lobato-Márquez D., Xu J., Güler G.Ö., Ojiakor A., Pilhofer M, & Mostowy S. (2021). Mechanistic insight into bacterial entrapment by septin cage reconstitution. Nature Communications, 12, 4511.

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Western Blot Analysis of Protein Samples

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Protein samples (purified or interaction complexes) or whole-cell fractions (cell extract after sonication or cell pellets) were dissolved in Laemmli sample buffer and boiled for 5 min. The protein samples loaded onto 12% or 15% SDS-PAGE gels were separated based on molecular weights, and transferred to a polyvinylidene difluoride membrane. The membrane was blocked with 5% nonfat dry milk in 1× Tris-buffered saline-Tween 20 (TBS-T) buffer and probed with anti-FLAG antibody (3:2,000 dilution, F1804; Sigma, St. Louis, MO, USA), anti-His₆ antibody (3:2,000 dilution, sc-8036; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-DnaK antibody (1:10,000 dilution, ADI-SPA-880; Enzo Life Science, Farmingdale, NY, USA) or anti-RpoB antibody (1:10,000 dilution, sc-56766; Santa Cruz Biotechnology, Santa Cruz, CA, USA) as primary antibodies. Anti-mouse IgG conjugated with peroxidase (3:5,000 dilution, sc-2005; Santa Cruz Biotechnology) was used as the secondary antibody in all Western blots. The chemiluminescent signals were developed with a West-Zol plus Western blot detection system (Intron Biotechnology, Seoul, South Korea).

Yoo W., Yoon H., Seok Y.J., Lee C.R., Lee H.H, & Ryu S. (2016). Fine-tuning of amino sugar homeostasis by EIIANtr in Salmonella Typhimurium. Scientific Reports, 6, 33055.

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