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Mouse 32 plex cytokine panel 1 kit

Manufactured by Merck Group
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The Mouse 32-Plex Cytokine Panel 1 kit is a multiplex assay designed to simultaneously measure the levels of 32 different cytokines and chemokines in mouse samples. The kit utilizes Luminex technology to provide a quantitative analysis of the target analytes.

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Lab products found in correlation

Map lysis buffer, by Merck Group (2 mentions)

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Mouse Cytokine 32-plex kit (2 citations)

2 protocols using mouse 32 plex cytokine panel 1 kit

1

Cytokine Profiling of Tumor Tissues

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2153L tumor tissues from the same batch with TMT MS were pulverized to powder under liquid nitrogen and lysed in MILLIPLEX MAP Lysis buffer (Millipore #43040). Lysates were cleared by centrifugation at 10,000 g three times at 4°C. The protein concentration of the supernatant was measured using a BCA Protein Assay Kit and adjusted to 1 mg/ml using lysis buffer. The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
Zhao N., Kabotyanski E.B., Saltzman A.B., Malovannaya A., Yuan X., Reineke L.C., Lieu N., Gao Y., Pedroza D.A., Calderon S.J., Smith A.J., Hamor C., Safari K., Savage S., Zhang B., Zhou J., Solis L.M., Hilsenbeck S.G., Fan C., Perou C.M, & Rosen J.M. (2023). Targeting EIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancer. bioRxiv.
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2

Cytokine Profiling of Tumor Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
2153L tumor tissues from the same batch with TMT MS were pulverized to powder under liquid nitrogen and lysed in MILLIPLEX MAP Lysis buffer (Millipore #43040). Lysates were cleared by centrifugation at 10,000 g three times at 4°C. The protein concentration of the supernatant was measured using a BCA Protein Assay Kit and adjusted to 1 mg/ml using lysis buffer. The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
Zhao N., Kabotyanski E.B., Saltzman A.B., Malovannaya A., Yuan X., Reineke L.C., Lieu N., Gao Y., Pedroza D.A., Calderon S.J., Smith A.J., Hamor C., Safari K., Savage S., Zhang B., Zhou J., Solis L.M., Hilsenbeck S.G., Fan C., Perou C.M, & Rosen J.M. (2023). Targeting EIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancer. bioRxiv.
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