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Nu702653

Manufactured by Bio-Techne
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The NU702653 is a compact and automated liquid handling system from Bio-Techne. It is designed for precise and reliable pipetting of various liquid volumes. The system features a motorized pipetting head that can aspirate and dispense liquids with high accuracy and repeatability.

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Lab products found in correlation

Ve 82139, by Axon Medchem (2 mentions) Ku5593351, by Bio-Techne (2 mentions) Dna pk, by Bio-Techne (2 mentions)

2 protocols using nu702653

1

Standardized Mouse Embryonic Fibroblast Culture

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
All MEFs used in this study were derived in house from day e13.5 embryos and grown in standard culture conditions as described21 (link). MEFs tested negative for mycoplasma contamination and genotypes were confirmed by PCR14 (link) and Western blot (Fig. 1c and Supplementary Fig. 1). Primary MEFs were immortalized by transfection with pBsSVD2005 (SV40 large T antigen expression vector). Adeno-Cre at a MOI of 500 was used. MEFs were grown for 3 days post-infection and split once prior to plating for experiments. Kinase inhibitors were used as follows: ATM, KU5593351 (link),52 (link) (10μM or 20 μM for 1-2 hrs as indicated, Tocris Biosciences); DNA-PK, NU702653 (link),54 (link) (20μM for 2 hrs, Tocris Biosciences); ATR, VE-82139 (10μM for 2 hrs, Axon Medchem).
Hartlerode A.J., Morgan M.J., Wu Y., Buis J, & Ferguson D.O. (2015). Recruitment and activation of the ATM kinase in the absence of DNA damage sensors. Nature structural & molecular biology, 22(9), 736-743.
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2

Standardized Mouse Embryonic Fibroblast Culture

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
All MEFs used in this study were derived in house from day e13.5 embryos and grown in standard culture conditions as described21 (link). MEFs tested negative for mycoplasma contamination and genotypes were confirmed by PCR14 (link) and Western blot (Fig. 1c and Supplementary Fig. 1). Primary MEFs were immortalized by transfection with pBsSVD2005 (SV40 large T antigen expression vector). Adeno-Cre at a MOI of 500 was used. MEFs were grown for 3 days post-infection and split once prior to plating for experiments. Kinase inhibitors were used as follows: ATM, KU5593351 (link),52 (link) (10μM or 20 μM for 1-2 hrs as indicated, Tocris Biosciences); DNA-PK, NU702653 (link),54 (link) (20μM for 2 hrs, Tocris Biosciences); ATR, VE-82139 (10μM for 2 hrs, Axon Medchem).
Hartlerode A.J., Morgan M.J., Wu Y., Buis J, & Ferguson D.O. (2015). Recruitment and activation of the ATM kinase in the absence of DNA damage sensors. Nature structural & molecular biology, 22(9), 736-743.
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