Poly ethylene glycol methacrylate pegma

All chemicals, reagents and organic solvents of the highest grade available were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated. The functional triethoxysilane BIB-APTES, bearing an ATRP initiating site, was obtained from a condensation reaction between 3-aminopropyltriethoxysilane (APTES) and 2-bromoisobutyrylbromide (BIBB) as previously reported^{41 (link)}. Poly(ethylene glycol) methacrylate (PEGMA) with a molar mass of 1100 g/mol and glycidyl methacrylate (GMA) were purchased from Sigma-Aldrich. Trypsin/Lys-C Mix Mass Spec Grade was provided by Promega (Madison, WI, USA). MilliQ water was prepared by arium 611 VF system from Sartorius (Göttingen, Germany).

All analytical grade reagents used in this study were purchased from Merck. These included AgNO₃(1mM), chloroform , ethanol, benzene, n-hexane, distilled water, 4-nitrophenol (4-NP), sodium borohydride and Triton X - 10 0. Poly (ethylene glycol) methacrylate (PEGMA, Mn∼526 g/mol), Formula:H₂C =C(CH₃)CO(OCH₂CH₂)nOH were bought from Sigma-Aldrich (Milwaukee, WI, USA). Fresh Caralluma tuberculata was collected in district Mardan, Pakistan. All glassware used in the preparation of all metal salt solutions and hybrid organic and inorganic matrices were cleaned with aqua regia and washed thoroughly with deionized water before use. All UV-visible spectra were measured with a Shimadzu UV-2550 spectrophotometer. Fourier transform infrared (FTIR) spectra were recorded using Nicolet 6700 FT-IR instrument (Thermo Scientific). The solution of nanoparticles and polymer capped were dropped onto copper grids to prepare specimens for transmission electron microscopic (TEM) observation which was performed in a JEM 2100F with a field-emission gun operating at 200 kV. Dynamic light scattering (DLS) study was done using a model BI-200SM instrument (Brookhaven Instrument Corporation) (Anwar et al., 2021 (link)).