R. officinalis and L. dentata were collected from Monterrey, N. L., Mexico. The specimens were identified in the Herbarium of the School of Biological Sciences, Universidad Autónoma de Nuevo Leon, Mexico. Dichloromethane (Baker, USA), methanol (Tedia, USA), acetone (CTR Scientific, USA), and isopropyl alcohol (Chromadex, USA) were of HPLC grade. Purified water was from a Milli-Q water-purification system (Veolia, USA). 1,8-cineol (>98%), β-pinene (98.5%), camphor (>95%), 4-allylanisole (98%), and linalool (>95%) were of GC grade (Aldrich, USA). The standard solution was n-alkanes (C8-C24, Sigma-Aldrich, USA). Eudragit EPO, an NC-formed polymer, was obtained from Evonik Industries, Germany.
N alkanes c8 c24
Manufactured by Merck Group
Sourced in United States
N-alkanes (C8-C24) are a class of saturated, linear hydrocarbon compounds. They consist of a chain of carbon atoms with hydrogen atoms attached. The range of carbon atoms spans from 8 to 24. These compounds are commonly used as reference standards and solvents in various analytical and chemical applications.
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Lab products found in correlation
Isopropyl alcohol, by ChromaDex (1 mentions)
β pinene, by Merck Group (1 mentions)
Camphor, by Merck Group (1 mentions)
Linalool, by Merck Group (1 mentions)
Methanol, by Tedia (1 mentions)
Eudragit epo, by Evonik (1 mentions)
Qp 2010 plus system, by Shimadzu (1 mentions)
Qp 2010 system, by Shimadzu (1 mentions)
Db 5ms column, by Shimadzu (1 mentions)
2 protocols using n alkanes c8 c24
1
Phytochemical Analysis of Rosemary and Lavender
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R. officinalis and L. dentata were collected from Monterrey, N. L., Mexico. The specimens were identified in the Herbarium of the School of Biological Sciences, Universidad Autónoma de Nuevo Leon, Mexico. Dichloromethane (Baker, USA), methanol (Tedia, USA), acetone (CTR Scientific, USA), and isopropyl alcohol (Chromadex, USA) were of HPLC grade. Purified water was from a Milli-Q water-purification system (Veolia, USA). 1,8-cineol (>98%), β-pinene (98.5%), camphor (>95%), 4-allylanisole (98%), and linalool (>95%) were of GC grade (Aldrich, USA). The standard solution was n-alkanes (C8-C24, Sigma-Aldrich, USA). Eudragit EPO, an NC-formed polymer, was obtained from Evonik Industries, Germany.
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R. officinalis and L. dentata were collected from Monterrey, N. L., Mexico. The specimens were identified in the Herbarium of the School of Biological Sciences, Universidad Autónoma de Nuevo Leon, Mexico. Dichloromethane (Baker, USA), methanol (Tedia, USA), acetone (CTR Scientific, USA), and isopropyl alcohol (Chromadex, USA) were of HPLC grade. Purified water was from a Milli-Q water-purification system (Veolia, USA). 1,8-cineol (>98%), β-pinene (98.5%), camphor (>95%), 4-allylanisole (98%), and linalool (>95%) were of GC grade (Aldrich, USA). The standard solution was n-alkanes (C8-C24, Sigma-Aldrich, USA). Eudragit EPO, an NC-formed polymer, was obtained from Evonik Industries, Germany.
2
Volatile Compounds Analysis of Cocoa Beans
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The fermented and dried cocoa beans were milled (A11B, Ika) and subjected to simultaneous distillation/extraction for 2 hours using 4 mL of pentane as a solvent. The volatile concentrate obtained was stored at a temperature of 5 °C [52 (link),53 (link),54 (link)].
Aliquots (2 μL) of volatile concentrate was injected into a gas chromatograph coupled to mass spectrometry (GC-MS), in a Shimadzu QP-2010 Plus system, equipped with a DB-5MS column (30 m × 0.25 mm × 0.25 μm). The helium gas was used as the carrier gas with a flow rate of 1.2 mL/min. The temperature of the injector and the interface was 250 °C, and the oven temperature was adjusted to 60–250 °C, using a ramp of 3 °C/min. Electronic impact mass spectrometer at 70 eV and the ion source temperature at 220 °C were used.
Quantitative analysis of the chemical constituents was performed by peak-area normalization using a flame ionization detector (FID—Shimadzu, QP 2010 system) under the same conditions as GC-MS, except that hydrogen was used as a mobile phase. Chemical identification was carried out by comparing the mass spectra and retention indices (RI) with those of standard substances in the system libraries and with data from the literature [55 ,56 ,57 ]. The RIs were obtained using a homologous series of n-alkanes (C8–C24, Sigma-Aldrich Co, St. Louis, MO, USA).
Aliquots (2 μL) of volatile concentrate was injected into a gas chromatograph coupled to mass spectrometry (GC-MS), in a Shimadzu QP-2010 Plus system, equipped with a DB-5MS column (30 m × 0.25 mm × 0.25 μm). The helium gas was used as the carrier gas with a flow rate of 1.2 mL/min. The temperature of the injector and the interface was 250 °C, and the oven temperature was adjusted to 60–250 °C, using a ramp of 3 °C/min. Electronic impact mass spectrometer at 70 eV and the ion source temperature at 220 °C were used.
Quantitative analysis of the chemical constituents was performed by peak-area normalization using a flame ionization detector (FID—Shimadzu, QP 2010 system) under the same conditions as GC-MS, except that hydrogen was used as a mobile phase. Chemical identification was carried out by comparing the mass spectra and retention indices (RI) with those of standard substances in the system libraries and with data from the literature [55 ,56 ,57 ]. The RIs were obtained using a homologous series of n-alkanes (C8–C24, Sigma-Aldrich Co, St. Louis, MO, USA).
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