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Whatman n 4

Manufactured by Cytiva
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Sourced in Switzerland, United Kingdom

Whatman No. 4 is a type of filter paper designed for general laboratory filtration applications. It has a medium-fine particle retention and is suitable for a variety of filtration tasks.

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Lab products found in correlation

Nylon filter, by GE Healthcare (1 mentions) R 210, by Büchi (1 mentions) Milli q system, by Waters Corporation (1 mentions) Bond elut nh2, by Agilent Technologies (1 mentions) Sodium azide nan3, by Merck Group (1 mentions) Sodium tetraborate decahydrate b4na2o7 10 h2o, by Merck Group (1 mentions) Whatman gf a, by Cytiva (1 mentions) Tween 20, by Merck Group (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions)

Close spelling variants of this product

Whatman filter paper n° 4 (11 citations) Whatman Paper N° 4 (4 citations)

6 protocols using whatman n 4

1

Heat-Assisted Extraction of R. ulmifolius Fruits

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Heat assisted extraction (HAE) was performed according to a methodology previously described by Pinela et al. [9 (link)], using 1.0 g of the R. ulmifolius fruit with 20 mL of solvent with different conditions previously defined by the RSM design (Table 1): time (t or X1, 20 to 120 min), temperature (T or X2, 20 to 90 °C) and ethanol proportions acidified with citric acid (pH 2.5) (S or X3, 0 to 100%). The solid-to-liquid ratio (S/L or X4) was maintained at 50 g/L. After the extraction, the samples were centrifuged (6000 rpm for 20 min at 10 °C) and filtered (paper filter Whatman nº 4) and the supernatant was collected and divided into two portions for HPLC-DAD and extraction yield analysis. A portion of 3 mL was filtered through a LC filter disk (nylon filter 0.2 µm, 25 mm diameter, Whatman™, GE Healthcare, Buckinghamshire, UK) for chromatographic analysis and another portion of 5 mL was dried in an incubator (Jouan, Berlim, Alemanha) at 105 °C over 48 h for suspended solids determination in order to determine the extraction yield assessment.
da Silva L.P., Pereira E., Prieto M.A., Simal-Gandara J., Pires T.C., Alves M.J., Calhelha R., Barros L, & Ferreira I.C. (2019). Rubus ulmifolius Schott as a Novel Source of Food Colorant: Extraction Optimization of Coloring Pigments and Incorporation in a Bakery Product. Molecules, 24(11), 2181.
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2

Acetone Extraction of Dry Biomass

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Dry biomass samples were extracted with 80% aqueous acetone (1:40, w/v) after stirring for 24 h, at room temperature (RT, approx. 20 °C). Extracts were filtered (paper Whatman nº4), solvent evaporated under reduced pressure (at 40 °C in rotary evaporator R-210, Buchi Labortechnik AG, Flawil, Switzerland), redissolved at 25 mg/mL in dimethyl sulfoxide (DMSO), and stored at −20 °C until needed.
Pereira C.G., Rodrigues M.J., Nawrot-Hadzik I., Matkowski A, & Custódio L. (2024). Seasonal and Geographic Dynamics in Bioproperties and Phytochemical Profile of Limonium algarvense Erben. Molecules, 29(2), 481.
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3

Biocontrol of Rice Blast Using Bacterial Isolates

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The isolates BRM 32111 and BRM 32112 were selected based on the results from the in vitro assays. One milliliter of bacterial suspension (10 8 ufc . ml -1 ) was transferred to Simmons liquid culture medium containing 3 × 10 5 conidia mL -1 of M. oryzae and kept in a shaker for 10 days. After centrifuging the resulting solutions, the supernatant was filtered using Whatman, nº 4 under vacuum.
Rice plants, at the V3 growth stage, were sprayed until the point of dripping, with a suspension containing conidia (3 × 10 5 mL -1 at final concentration) of M. oryzae mixed with: T1-control (water), T2-cell suspension of BRM 32111, T3-cell suspension of BRM 32112, T4-filtrate of BRM 32112, T5-filtrate BRM 32111, T6-filtrate of BRM 32111 + M. oryzae conidia, T7-filtrate of BRM 32112 + M. oryzae. After spray inoculations, the plants were incubated at temperatures varying from 28 to 30 ºC at high humidity.
Martins B.E.D.M., Chaibub A.A., Côrtes M.V.d.C.B., Lobo V.L.d.S., & Filippi M.C.C.d. (2020). CHARACTERIZATION OF BACTERIAL ISOLATES FOR SUSTAINABLE RICE BLAST CONTROL. Revista Caatinga, 33(3), 702-712.
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4

Isolation of Vochysiamides A and B

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Strain LGMF1583 was cultivated in PDA at 28 °C for 3 days, and culture was cut into 8 mm plugs. Five plugs were used to inoculate 32 Erlenmeyer flasks (500 mL) containing 250 mL MEB medium and incubated at 28 °C for 10 days on a rotary shaker (180 rpm). After this period, the culture was filtered-off on Whatman n. 4, and the supernatant was extracted with ethyl acetate (3 x v/v). The recovered organic extracts were evaporated in vacuo at 40 °C to yield 850 mg of a brown, oily crude extract (Fig. 1).
As highlighted in Fig. 1, the crude extract obtained (850 mg) was subjected to a Reverse Phase C18 column chromatography (20 × 2.0 cm, 250g) eluted with a gradient of H2O- CH3CN (100:0 - 0:100) followed by HPLC analysis to produce five fractions [FI (187 mg), FII (135 mg), FIII (191 mg), FIV (83 mg) and FV (171 mg)]. Further purification of FIII using Sephadex LH-20 (MeOH, 25.0 × 2.0 cm) followed by HPLC afforded the new compounds, vochysiamides A (1; 4.0 mg) and B (2; 7.0 mg) in pure forms as white solids. In the same manner, FI was subjected to Sephadex LH-20 (CH2Cl2/40% MeOH; 1 × 40 cm), followed by HPLC purification to afford 2,5-dihydroxybenzyl alcohol (3; 36.2 mg) in pure form as white solid (Fig. 1). All the remaining fractions and sub-fractions were omitted based on the HPLC analysis and/or the absence of bioactivity.
Noriler S.A., Savi D.C., Ponomareva L.V., Rohr J., Thorson J.S., Rodrigues R., Glienke C, & Shaaban K.A. (2019). Vochysiamides A and B: Two new bioactive carboxamides produced by the new species Diaporthe vochysiae. Fitoterapia, 138, 104273.
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5

Aflatoxin Extraction and Quantification

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OTA, sodium tetraborate decahydrate (B4Na2O7 × 10 H2O), TWEEN® 20, sodium azide (NaN3) and ovalbumin (OVA) were obtained from Sigma-Aldrich (Milan, Italy). MAb clone 5E2 (Order No. 201052) was provided by Softflow Biotechnology (Pécs, Hungary). Glass culture tubes (10 × 75 mm) were supplied by VWR International S.R.L. (Milan, Italy). Glass microfiber filters (Whatman GF/A) and paper filters (Whatman N. 4) were purchased from Whatman (Maidstone, UK). Solid phase extraction columns Bond Elut NH2 (500 mg, 3 mL) were purchased by Agilent Technologies (Santa Clara, CA, USA). OchraTest™ immunoaffinity columns were provided by VICAM, a Water Business (Milford, MA, USA). All other chemicals and solvents were reagent grade or HPLC grade and were obtained from Sigma-Aldrich (Milan, Italy). Ultrapure water was produced by a Waters Milli-Q system (Waters, Milford, MA, USA).
Lippolis V., Porricelli A.C., Cortese M., Suman M., Zanardi S, & Pascale M. (2017). Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay. Toxins, 9(10), 305.
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6

Extraction of Tannat Grape Pomace

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Seeds and skins of Tannat grape pomace were manually separated and the skin was submitted to drying at 40 °C up to constant weight, achieved at 24 h [3 (link)]. Skin was then powdered by employing a domestic mill.
Extract was prepared by weighting 20 g of Tannat dried grape skin powder and adding 200 mL of solvent (methanol-water-formic acid, 70:25:5) (hydro-alcoholic-acid extraction, EHAA). The extract (EHAA) was then filtered through filter paper (Whatman n°4), the supernatant dried in a rotary evaporator, then diluted with distilled water, freeze dried for 4 days, and stored at −20 °C for subsequent analysis.
Fernández-Fernández A.M., Iriondo-DeHond A., Nardin T., Larcher R., Dellacassa E., Medrano-Fernandez A, & del Castillo M.D. (2020). In Vitro Bioaccessibility of Extractable Compounds from Tannat Grape Skin Possessing Health Promoting Properties with Potential to Reduce the Risk of Diabetes. Foods, 9(11), 1575.
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