Tecnai g2 f20 s twin microscope

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Tecnai G2 F20 S-Twin microscope is a high-performance, field-emission transmission electron microscope designed for advanced imaging and analytical applications. It features a unique S-Twin lens configuration, providing enhanced resolution and contrast for a wide range of sample types.

Automatically generated - may contain errors

Lab products found in correlation

Escalab 250 spectrometer, by Thermo Fisher Scientific (4 mentions) Digital micrograph 3, by Ametek (3 mentions) Gimp 2, by Ametek (2 mentions) Verious 460 l scanning electron microscope, by Thermo Fisher Scientific (2 mentions) Nicolet istm10, by Thermo Fisher Scientific (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Sem 5500, by Hitachi (1 mentions) D8 advance, by Bruker (1 mentions) Ultima 4 diffractometer, by Rigaku (1 mentions) Tecnai g2 f20 s twin transmission, by Thermo Fisher Scientific (1 mentions)

10 protocols using tecnai g2 f20 s twin microscope

Characterization of Biosynthesized Quantum Dots

Check if the same lab product or an alternative is used in the 5 most similar protocols

The size of biosynthesized QDs was determined by dynamic light scattering (DLS). Purified and concentrated QDs were sonicated for 2 min and then measured in triplicate using a Zetasizer Nano (ZS) (Malvern Instrument Ltd.). High resolution scanning transmission electron microscopy (HR-STEM) was used to confirm nanometric size and chemical composition (FEI Tecnai G2 F20 S-Twin microscope, operated at 200 kV). For these purposes, 2 μL of the purified and concentrated QDs solution was added to a HC300-Cu grid and left to dry. TEM images were processed and analyzed with Digital Micrograph 3.9.0 (Gatan Inc) and The Gimp 2.4.0 software packages. In addition, samples were chemically characterized by Energy-dispersive X-ray spectroscopy (EDS or EDX).
To determine the organic composition of the external layer of QDs, samples werefreeze-dried for 48 h and the powder obtained was mixed with KBr to form a thin pellet. FTIR spectroscopy in a range between 600 and 4000 cm^–1 was performed using a Nicolet^TM iS^TM10 (Thermo Fisher Scientific Inc.).

Órdenes-Aenishanslins N., Anziani-Ostuni G., Quezada C.P., Espinoza-González R., Bravo D, & Pérez-Donoso J.M. (2019). Biological Synthesis of CdS/CdSe Core/Shell Nanoparticles and Its Application in Quantum Dot Sensitized Solar Cells. Frontiers in Microbiology, 10, 1587.

+ Open protocol

+ Expand

Characterization of Advanced Composites

Check if the same lab product or an alternative is used in the 5 most similar protocols

The density of the specimens was examined using the Archimedes method (PN-EN 1094-4:1998 Standard [36 ]). Hardness was measured with the Vickers Hardness Tester (FV-700e, Future-Tech, Kawasaki, Japan) using the indentation method under the load of 49.05 N. The fracture toughness of the produced composites was determined with the Vickers indentation crack length method under the load of 49.05 N. The Niihara, Morena, Hasselman equation was used. A total of 20 hardness measurements and 12 crack length measurements were performed for each sample. The phase composition of composites was analyzed with X-ray diffraction (XRD, D8 ADVANCE, Bruker, Billerica, MA, USA), using CuKα radiation at a wavelength of λ = 0.154056 nm. The parameters of this test were as follows: voltage: 40 kV, current: 40 mA, angular range: 20–100° with step 0.05°. The microstructure observations were performed on a scanning electron microscope (SEM Hitachi 5500, Hitachi, Tokyo, Japan). The observations were carried out at a 20 kV accelerating voltage. The scanning transmission electron microscopy images and diffraction patterns were obtained on an FEI Tecnai G2 F20 S-TWIN microscope (FEI, Hillsboro, OR, USA), operating at 200 kV and equipped with a Fischione 3000 high angle annular dark-field (HAADF) STEM detector.

Petrus M., Woźniak J., Cygan T., Lachowski A., Moszczyńska D., Adamczyk-Cieślak B., Rozmysłowska-Wojciechowska A., Wojciechowski T., Ziemkowska W., Jastrzębska A, & Olszyna A. (2021). Influence of Ti3C2Tx MXene and Surface-Modified Ti3C2Tx MXene Addition on Microstructure and Mechanical Properties of Silicon Carbide Composites Sintered via Spark Plasma Sintering Method. Materials, 14(13), 3558.

+ Open protocol

+ Expand

Nanoparticle Characterization by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

TEM measurements were performed using a FEI Tecnai G2 F20 S-Twin microscope, operated at 200 kV. A drop of the dispersed sample was left to dry out on a commercial carbon coated Cu TEM grid. TEM images were processed and analyzed with Digital Micrograph 3.9.0 (Gatan Inc) and The Gimp 2.4.0 software packages. A statistical study of TEM images was carried out to quantify nanoparticles sizes. This study consists of the size measurement (diameter) of about 200 particles per sample. The counts were then plotted as frequency histograms and the mean particle size was calculated. In addition, samples were chemically characterized by Energy-dispersive X-ray spectroscopy (EDX) and electron diffraction (ED).

Bruna N., Collao B., Tello A., Caravantes P., Díaz-Silva N., Monrás J.P., Órdenes-Aenishanslins N., Flores M., Espinoza-Gonzalez R., Bravo D, & Pérez-Donoso J.M. (2019). Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria. Scientific Reports, 9, 1953.

+ Open protocol

+ Expand

Multiscale Characterization of Nanocomposites

Check if the same lab product or an alternative is used in the 5 most similar protocols

SEM images were measured on FEI Verious 460 L scanning electron microscope (Hillsboro, OH, USA). AFM images were investigated by Icon Bruker microscope (Ettlingen, Germany). TEM images were measured on a FEI Tecnai G2F20 S-TWIN microscope equipped with an energy-dispersive spectrometer (EDS) (Hillsboro, OH, USA). XPS analysis were measured on a Thermo ESCALAB 250 spectrometer (Shanghai, China). The EC and capacitive properties of the films were determined by combining the in-situ TU-1901 PERSEE UV-vis spectrophotometer (Beijing, China) with an CHI660B Chenhua electrochemical workstation (Shanghai, China) in a three-electrode configuration, where the nanocomposites served as the working electrodes; a Pt plate/Pt wire acted as the counter electrode, and Ag/AgCl was used as the reference electrode.

Fu Y., Yang Y., Chu D., Liu Z., Zhou L., Yu X, & Qu X. (2022). Vanadium-Substituted Dawson-Type Polyoxometalate–TiO2 Nanowire Composite Film as Advanced Cathode Material for Bifunctional Electrochromic Energy-Storage Devices. Molecules, 27(13), 4291.

+ Open protocol

+ Expand

Negative Staining of DNA Nanostructures

Check if the same lab product or an alternative is used in the 5 most similar protocols

5 μL of sample containing
DNA objects at a concentration of 2–10 nM was pipetted onto
a plasma-treated carbon grid. The sample droplets were incubated on
the grids for 3 min and then blotted away using filter paper. A 5
μL droplet of 2% aqueous uranyl formate (UFO) solution containing
25 mM sodium hydroxide was pipetted onto the grid and immediately
blotted away. Subsequently, a 20 μL UFO droplet was pipetted
onto the grid, incubated for 30 s, and blotted away. The grids were
air-dried for 20 min before imaging on an FEI Tecnai G2 F20 S-TWIN
microscope at 200 kV.

Li W., Wang Z., Su Q., Chen J., Wu Q., Sun X., Zhu S., Li X., Wei H., Zeng J., Guo L., Zhang C, & He J. (2024). A Reconfigurable DNA Framework Nanotube-Assisted Antiangiogenic Therapy. JACS Au, 4(4), 1345-1355.

+ Open protocol

+ Expand

Transmission Electron Microscopy of Dispersed Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transmission electron microscopy (TEM) measurements were made using a FEI Tecnai G2 F20 S-Twin microscope, operated at 200 kV. For these studies, a drop of the dispersed sample was left to dry out on a commercial carbon coated Cu TEM grid. TEM images were processed and analyzed with Digital Micrograph 3.9.0 (Gatan Inc) and The Gimp 2.4.0 software packages. In addition, samples were chemically characterized by Energy-dispersive X-ray spectroscopy (EDS or EDX).

Ulloa G., Quezada C.P., Araneda M., Escobar B., Fuentes E., Álvarez S.A., Castro M., Bruna N., Espinoza-González R., Bravo D, & Pérez-Donoso J.M. (2018). Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidans ATCC 19703 by Improving Metal Uptake and Tolerance. Frontiers in Microbiology, 9, 234.

+ Open protocol

+ Expand

Comprehensive Material Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transmission electron
microscopy (TEM) analysis was carried out using a FEI Tecnai G2 F20S-Twin
microscope at a 200 kV accelerating voltage. For sample preparation,
the powders were dispersed in an ethanol solution through sonication,
and then one drop of the suspension was dropped onto a microgrid.
Fourier transform infrared (FTIR) spectra were obtained using a Nicolet
iS5 spectrophotometer (frequency range from 4000 to 500 cm^–1) with a KBr pellet. Thermogravimetric analysis (TGA) was performed
on a Setaram Evolution 16/18 apparatus. The samples were heated in
an alumina pan from 30 to 800 °C at a heating rate of 10 °C/min
under a high-purity nitrogen atmosphere. The N₂ adsorption–desorption
isotherms were obtained on a Quantachrome autosorb iQ₂ analyzer.
Before measurement, the samples were first degassed under vacuum at
393 K for 6 h at a heating rate of 5 °C/min. The specific surface
areas of the samples were calculated using the Brunauer–Emmet–Teller
(BET) method; pore size distribution and pore volume were calculated
using the Barrett–Joyner–Halenda (BJH) model.
X-ray diffraction (XRD) measurements were recorded on a Rigaku Ultima
IV diffractometer using Cu-Kα radiation as the X-ray source
in the 2θ range of 10°–80°. The X-ray photoelectron
spectra (XPS) were recorded on the PHI-5702 instrument, and the C_1s line at 284.5 eV was used as the binding-energy reference.

Zhang F., Li J., Li X., Yang M., Yang H, & Zhang X.M. (2016). In Situ Surface Engineering of Mesoporous Silica Generates Interfacial Activity and Catalytic Acceleration Effect. ACS Omega, 1(5), 930-938.

+ Open protocol

+ Expand

TEM Analysis of Nanostructured Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols

TEM and HRTEM images and electron diffraction were performed on a FEI Tecnai G2 F20 S-Twin microscope operated at an accelerating voltage of up to 200 kV. The TEM samples were prepared by sonication at 500 W for ∼5 min and 25 μl of the supernatant were dropped onto holey carbon grids.

Geng X., Sun W., Wu W., Chen B., Al-Hilo A., Benamara M., Zhu H., Watanabe F., Cui J, & Chen T.P. (2016). Pure and stable metallic phase molybdenum disulfide nanosheets for hydrogen evolution reaction. Nature Communications, 7, 10672.

+ Open protocol

+ Expand

Multimodal Characterization of Thin Films

Check if the same lab product or an alternative is used in the 5 most similar protocols

FTIR spectroscopy was conducted using a GangDong FTIR-650 spectrometer (Tianjin, China) between 400 and 4000 cm⁻¹. SEM images were measured on FEI Verious 460 L scanning electron microscope (Hillsboro, OH, USA). AFM images were investigated by Icon Bruker microscope (Ettlingen, Germany). TEM images were measured on a FEI Tecnai G2F20 S-TWIN microscope equipped with an energy-dispersive spectrometer (EDS) (Hillsboro, OH, USA). The TEM sample was prepared by scraping a small amount of the film (0.005 mg) off the FTO substate and dispersing the powder in 1 mL of an ethanol solution by ultrasonication for 40 min. XPS analysis were measured on a Thermo ESCALAB 250 spectrometer (Shanghai, China).

Qu X., Zhou L., Liu Z., Wang Z., Wang J., Yu X., Jin H, & Yang Y. (2023). Sea-Cucumber-like Microstructure Polyoxometalate/TiO2 Nanocomposite Electrode for High-Performance Electrochromic Energy Storage Devices. Molecules, 28(6), 2634.

+ Open protocol

+ Expand

Fimbriae Reconstruction and Visualization by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Using TEM to observe the reconstruction of fimbriae in vitro, the concentrations of purified mtCfaA-CfaE, mtCfaA-CfaB and CfaC are 0.25 μM, 5 μM and 0.25 μM, respectively, each sample was incubated at 25°C for 24 hours in a buffer containing 10 mM Tris-HCl, pH 7.5 and 20 mM NaCl, negatively stained with 2% (w/v) uranyl acetate for 1 min and imaged using FEI Tecnai G2 F20 S-TWIN microscope. The micrographs were recorded at an accelerating voltage of 200 kV and a magnification of 20,000. To illustrate the effect of different mutations of CfaE on CFA/I fimbriation by TEM, sample cell pastes were resuspended in PBS, pH 7.4 to a density of 107–108 cells mL-1. Formvar/carbon coated 300 mesh copper grids were prepared by adsorbing 2–5 μL bacterial suspensions for 5 minutes. The grids were then negatively stained with 2% uranyl acetate for 1–2 minutes and imaged using a FEI Tecnai G2 F20 S-TWIN transmission electron microscope. Micrographs were recorded at an accelerating voltage of 200 kV.

He L.H., Wang H., Liu Y., Kang M., Li T., Li C.C., Tong A.P., Zhu Y.B., Song Y.J., Savarino S.J., Prouty M.G., Xia D, & Bao R. (2020). Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis. PLoS Pathogens, 16(10), e1008848.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!