In this study, DF microscopy imaging was used under a Nikon inverted microscope (ECLIPSE Ti-U). In DF mode, the microscope utilized a Nikon Plan Fluor 100× 0.5–1.3 oil iris objective and a Nikon DF condenser. An Andor iXonEM + CCD camera (iXon Ultra 897) was employed to record DF images of Ag@AuNRs. The collected images were analyzed with the Image J software. Furthermore, DF scattering spectra were acquired with an Andor spectrophotometer (SHAMROCK 303i, SR-303I-A) and an Andor CCD camera (Newton DU920P-OE). When recording a spectrum, the scanning stage moved the sample to the desired location was collected by the objective. The scattered light was directed to the entrance of the spectrometer, dispersed by a grating (300 L mm−1) and detected by the Newton CCD camera. The background was measured at a region without nanoparticles. Data analysis on the experimental data was performed by Matlab programs specially designed for this study.
Newton du920p oe
Manufactured by Oxford Instruments
The Newton DU920P-OE is a scientific instrument designed for spectroscopic analysis. It features a thermoelectrically cooled and back-illuminated charge-coupled device (CCD) sensor for high-sensitivity detection. The device is capable of spectral measurements across a wide range of wavelengths.
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Lab products found in correlation
2 protocols using newton du920p oe
1
Plasmonic Nanoparticle Optical Characterization
2
Steady-State Absorption and Phosphorescence Spectra
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Steady-state absorption spectra were measured with an UV-3600 Plus (SHIMADZU) at room temperature with 1 cm quartz cuvette. Phosphorescence spectra were measured by home-build millisecond time-resolved emission spectrometer at 77 K with nitrogen cryostat (OptistatDN2, Oxford instruments). Briefly, the cooled samples in EPA (diethyl ether/isopentane/ethanol 5:5:2) under argon atmosphere were excited with a 355-nm continuous wave (CW) laser (Genesis CX355 100SLM AO, Coherent) and the emission was detected by EMCCD (Newton DU920P-OE, Andor Technology). The excitation light was blocked with 1 Hz by an optical shutter (76992 and 6995, ORIEL) and the time evolution of the emission spectra was measured to separate the fluorescence and phosphorescence. The shutter was controlled by LabVIEW.
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