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2 protocols using hbx antibody

1

Antibody Procurement for Cellular Experiments

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FLAG-tag and β-actin antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). GST, His-tag, HIF-2α, fibronectin, Slug, Snail and vimentin antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). HA-tag antibody was purchased form AbFrontier (Seoul, Korea). VHL, HIF-1α, E-cadherin, occluding, and Ki-67 antibodies were purchased from BD Pharmingen (San Jose, CA, USA). HBx antibody was purchased from abcam (Cambridge, MA, USA). The proteasome inhibitor, MG132 (#C2211) and protein synthesis inhibitor, cycloheximide (#01810), were purchased from Sigma-Aldrich. The list of antibodies is described in Supplementary Table 1. The anti-UCP antibody was generated as reported previously [15 (link)].
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2

Antibody Validation and Inhibitor Sourcing

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TRIM52 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HBx antibody was obtained from Abcam (Cambridge, UK). NF-κB p65 and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Pyrrolidine dithiocarbamate (PDTC) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
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