Cepharanthine was bought from Aladdin Chemistry Co., Ltd (C102706-5q), solubilized in DMSO, and stored at −20°C for later use in experiments. DMEM/F12 medium, FBS, pancreatic enzyme, and streptomycin/penicillin were acquired from Gibco (NY, United States). Collagenase II and DMSO were bought from Sigma-Aldrich (Merck KGaA, MO, United States). 17β-oestradiol (E2) was obtained from Selleck (Shanghai, China). Recombinant IL-1β and TNF-α were obtained from R&D Systems (Abingdon, United Kingdom). The radio-immuno precipitation assay (RIPA) lysis buffer and the bicinchoninic acid (BCA) assay kit were purchased from Hangzhou Fude Biological Technology Co., Ltd (Hangzhou, China). Also, 3-methyladenine (3-MA) and anisomycin were obtained from MedChemExpress (Monmouth Junction, NJ).
Pancreatic enzyme
Manufactured by Thermo Fisher Scientific
Sourced in United States
Pancreatic enzymes are a group of enzymes produced by the pancreas. These enzymes play a crucial role in the digestion and breakdown of various macromolecules, including fats, proteins, and carbohydrates, in the human digestive system.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Collagenase 2, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Bupivacaine, by Merck Group (2 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Recombinant rat il 1β, by R&D Systems (2 mentions)
Rbc lysis buffer, by Thermo Fisher Scientific (2 mentions)
Octadecylamine, by Merck Group (2 mentions)
Anisomycin, by MedChemExpress (1 mentions)
Recombinant il 1β, by R&D Systems (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 methyladenine 3 ma, by MedChemExpress (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Type 2 collagen enzyme, by Merck Group (1 mentions)
Toluidine blue, by Solarbio (1 mentions)
15 protocols using pancreatic enzyme
1
Cepharanthine Modulation of Inflammation
2
Isolation and Characterization of Chondrocytes
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The cartilage collected from the OA patients in the surgery was cut into sections sized 1 ~ 2 mm3, followed by PBS washing and 0.25% type II collagen enzyme (Sigma-Aldrich Chemical Company) digestion overnight. The digestive juice was filtered by a 200 mesh cell filter (Corning Glass Works, Corning, N.Y., USA), and then the chondrocytes were centrifuged at 1000g for 5 min. After discarding the supernatant, the cells were added with Dulbecco minimum essential medium (DMEM) containing 10% fetal bovine serum (FBS) (Gibco Company, Grand Island, NY, USA), followed by suspension and culture at 37°C with 5% CO2. After growing full of the incubator, the chondrocytes were digested with 0.25% pancreatic enzyme (Gibco Company) and cultured for passage. Well-grown chondrocytes were collected to make climbing films, which were stained with toluidine blue (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) according to the reagent specification of immunochemistry staining, and labelled with type II collagen (USCN Life Science & Technology Company, Double Lake, Missouri City, TX, USA), followed by dehydration with absolute alcohol, mounting with neutral balsam and observing under a microscope and picture-taking.
3
Culturing Rat Hepatocyte Cell Line
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Normal rat hepatocyte cell line BRL was purchased from the Cell Research Center of Chinese Academy of Science (Shanghai, China). The cells were maintained with DMEM containing 10% new-born calf serum, 0.25% pancreatic enzyme (Gibco, Grand Island, USA), 100 ng/mL of penicillin, and 100 ng/mL of streptomycin at 37°C in a humidified atmosphere containing 5% CO2.
4
Bupivacaine's Effects on Cellular Viability
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The Bupivacaine was purchased from Sigma-Aldrich, USA. DMEM/F12, fetal calf serum, and pancreatic enzyme (including or excluding) EDTA were purchased from Gibco, USA. PJ34 HCl was purchased from Selleck. Bupivacaine, 2,7-dichlorofluorescein diacetate (DCFH-DA), dihydroethidium (DHE) dye were purchased from Sigma-Aldrich, USA.
5
Bupivacaine Cytotoxicity on SH-SY5Y Cells
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Bupivacaine was purchased from Sigma, USA. DMEM-F12, fetal calf serum and pancreatic enzyme (including or excluding) EDTA were purchased from Gibco, USA. SH-SY5Y cells were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China).
6
Spironolactone-mediated Inflammation Modulation
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Spironolactone was purchased from Sigma, USA (CAS50-01-7). DMEM, FBS, streptomycin, penicillin, and 0.25% pancreatic enzyme were all obtained from Gibco, NY, USA. Recombinant rat IL-1β was purchased from R&D Systems (Abingdon, UK), and collagenase II, dimethyl sulfoxide, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The bicinchoninic acid assay kit and radioimmunoprecipitation assay (RIPA) buffer were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Asiatic acid and betulinic acid were purchased from Selleck Chemicals.
7
Chondrogenic, Osteogenic, Adipogenic Differentiation
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Fetal bovine serum (FBS), streptomycin, penicillin, 0.25% pancreatic enzyme and DMEM were obtained from Gibco, USA. Chondrogenic induction medium, osteogenic induction medium and adipogenic induction medium were obtained from Cyagen US Inc. Hematoxylin and BSA were obtained from Sigma-Aldrich, USA. Mitochondrial fusion promoter M1 was obtained from Selleckchem (Shanghai, China).
8
Madecassoside Cytokine-Mediated Cell Assay
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Madecassoside was purchased from Nantong Feiyu Biological Technology, Co., Ltd. DMEM, FBS, streptomycin, penicillin, and 0.25% pancreatic enzyme were all obtained from Gibco, NY, USA. Recombinant rat IL-1β was purchased from R&D Systems, Abingdon, UK, and collagenase II, DMSO, and BSA were obtained from Sigma-Aldrich, Merck KGaA, MO, USA. The bicinchoninic acid (BCA) assay kit and RIPA buffer were purchased from the Beyotime Institute of Biotechnology, Shanghai, China.
9
Synthesis and Characterization of Biomaterial Composites
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Poly(ethylene oxide) (PEO; MW = 2 kDa), glycol chitosan (GC, MW ∼ 250 kDa), and sodium alginate (MW ∼ 150 kDa, M/G ratio ∼ 1.6) were purchased from Sigma-Aldrich (St. Louis, United States). Benzaldehyde-capped poly(ethylene oxide) (CHO-PEO-CHO) and silica nanoparticles (average diameter of 12 nm) were synthesized according to our previous reports (Ding et al., 2010 (link); Zhu et al., 2016 (link), 2017 (link)). BG particles were synthesized according to our previous work (Ji et al., 2017 (link)). RPMI1640 medium, pancreatic enzymes, fetal bovine serum (FBS), penicillin-streptomycin solution, and phosphate-buffered saline (PBS) were purchased from Gibco (Grant Island, United States).
10
Thyroid Tissue Single-Cell Preparation
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We processed thyroid tissues immediately after resection and generated single-cell suspensions. To prepare single-cell suspension, the dissected tissues were washed with Hanks’ balanced salt solution (HBSS) and sheared on ice. Then, the tissues were digested by collagenase I (2 mg/ml) (Gibco 1710-0017), collagenase IV (1 mg/ml) (Gibco 1710-4019), and 0.25% pancreatic enzymes (Gibco 25200-056) for 1 hour at 37°C. The digested tissues were filtered through 40-mm strainer to remove cell debris and large clumps. The suspensions were pelleted at 500g for 5 min at 4°C. After lysis of the red blood cells with 10× RBC Lysis Buffer (Thermo Fisher Scientific, 00-4300-54), the pellets were resuspended in HBSS with 0.04% bovine serum albumin (BSA) to count cell viability and concentration (Counting Star, Aber Instruments Ltd.). The remaining cells were pelleted at 500g for 5 min at 4°C and preserved at −80°C. Single-cell suspensions were finally diluted to 5 × 106 cells per milliliter. All tissues were acquired from West China Biobanks, Department of Clinical Research Management, West China Hospital, Sichuan University.
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