MiR-223-5p mimics, NC mimics and miR-223-5p inhibitors were purchased from Ribobio (Guangzhou, China). HVSMCs were seed into a 24-well plate at density of 1 × 105 cells/well and cultured overnight. On the next day, the cells were divided into five groups: blank group, NC group, Exos group, miRNA mimics group and miRNA inhibitor + Exos group. The cells in the NC group and miRNA mimics group were, respectively, transfected with NC mimics and MiR-223-5p mimics using Lipofectamine 2000 (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions. The cells in the Exos group were treated with 100 µg TAO plasma-derived exosomes. The cells in the miRNA inhibitor + Exos group were firstly transfected with miR-223-5p inhibitor using Lipofectamine 2000 and then were treated with 100 µg TAO plasma-derived exosomes. The cells in the blank group were without treatments.
Mir 223 5p inhibitors
Manufactured by RiboBio
Sourced in China
MiR-223-5p inhibitors are a type of laboratory equipment used in molecular biology and genetics research. These inhibitors work by targeting and suppressing the activity of the microRNA molecule miR-223-5p, which plays a role in various cellular processes. The core function of MiR-223-5p inhibitors is to facilitate the study of the biological functions and regulatory mechanisms of miR-223-5p in different experimental systems.
Automatically generated - may contain errors
2 protocols using mir 223 5p inhibitors
1
Exosomal miR-223-5p Regulates HVSMC
2
Exosomal miR-223-5p Regulates HVSMC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MiR-223-5p mimics, NC mimics and miR-223-5p inhibitors were purchased from Ribobio (Guangzhou, China). HVSMCs were seed into a 24-well plate at density of 1 × 105 cells/well and cultured overnight. On the next day, the cells were divided into five groups: blank group, NC group, Exos group, miRNA mimics group and miRNA inhibitor + Exos group. The cells in the NC group and miRNA mimics group were, respectively, transfected with NC mimics and MiR-223-5p mimics using Lipofectamine 2000 (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions. The cells in the Exos group were treated with 100 µg TAO plasma-derived exosomes. The cells in the miRNA inhibitor + Exos group were firstly transfected with miR-223-5p inhibitor using Lipofectamine 2000 and then were treated with 100 µg TAO plasma-derived exosomes. The cells in the blank group were without treatments.
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