The Folin-Ciocalteu protocol [21 (link)] was used for the evaluation of total phenolic compounds in selected parts of plants. For the experiment, 1000 μL of extracts was added to 5 ml of Folin-Ciocalteu reagent (mixed with distilled water 1 : 10 v/v) and 4 ml (75 g/L) of sodium carbonate. The samples were vortexed for 15 s and left to stand for 30 min at 40°C for staining. The absorbance was determined at a wavelength of 765 nm using the Thermo Scientific Spectrascan UV 2700 dual-beam spectrophotometer. The concentration of each plant extract was 0.1 g/ml. Phenol levels were calculated in mg/g of n-propyl gallate equivalent.
Spectrascan uv 2700
Manufactured by Thermo Fisher Scientific
Sourced in India
The Spectrascan UV 2700 is a double-beam UV-Vis spectrophotometer designed for accurate and reliable measurements in a wide range of applications. It features a wavelength range of 190 to 1100 nm and can perform various spectroscopic analyses including absorbance, transmittance, and reflectance measurements.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using spectrascan uv 2700
1
Folin-Ciocalteu Assay for Plant Phenolics
2
Optical Analysis of Biosynthesized Silver Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To observe the optical property of biosynthesized silver nanoparticles, samples were periodically analyzed with UV-vis spectroscopic studies (Thermo Scientific Spectrascan UV 2700) at room temperature operated at a resolution of 1 nm between 200 and 600 nm ranges.
3
Biofilm Formation Assay for Phosphate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two ml of NBRIY broth (pH 7.0) supplemented with different concentrations of K2HPO4 were taken in separate wells of 24 wells polystyrene cell culture plates and were inoculated with 20 μl of bacterial culture (OD620nm = 0.5). Uninoculated wells for each concentration were treated as control. All the plates were then kept undisturbed in a BOD incubator (Instrumentation India, Kolkata, India) at 28 °C for 48 hours. After that broth were decanted from each well without disturbing the biofilms and the wells were washed twice with sterilized distilled water carefully to remove the planktonic cells. After air drying in front of laminar airflow, Two ml of 0.1% crystal violet solution was added to each well55 (link). After 10 minutes of incubation at room temperature, the stain was removed from the wells and the plates were finally washed with sterilized distilled water for 3–4 times and again air dried. One ml of 33% acetic acid was applied to each well and then incubated for 30 minutes for extracting the crystal violet from adhered cells56 . Optical densities were then measured at 595 nm using UV-VIS spectrophotometer (SPECTRASCAN UV-2700, Thermo Scientific, India). For confirming the role of P concentration on biofilm formation process, similar quantification experiments were also performed by replacing the P source (K2HPO4) with Na2HPO4.
4
Catalytic Effect of Biogenic GNPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Effect of different shapes and sizes of biosynthesized GNPs on their catalytic activity to reduce 4NP into 4AP was assessed by the method of Gangula et al.42 (link), with some minor modifications. In a 3 ml of quartz cuvette, 1.7 mL of water, 0.3 mL of 2 mM solution of 4-nitrophenol and 1 ml of 0.03 M of freshly prepared NaBH4 solution were added43 (link)44 (link). To this reaction mixture, 50 μL of gold nanoparticles of different morphologies viz. spherical, triangular, hexagonal and sheet shaped were added. The reaction temperature was kept constant at room temperature (25 °C) to avoid the thermal effect on the process of catalysis. The reaction mixture was stirred well with microstirrer and quickly scanned between 200–600 nm in UV-vis spectroscopy (Thermo spectrascan UV 2700). Further, to assess the effect of size, spherical gold nanoparticles of two different size range (3–10 and 7–24 nm) were used as biocatalyst. The 4-nitrophenol shows an absorbance peak at 400 nm in presence of NaBH4 due to formation of nitrophenolate ions, so the progress of the reaction was monitored by tracking the decrease in the absorption spectra of 4-nitrophenolate ion at 400 nm. Kinetics of the reaction was monitored for 15 min which showed a decrease in absorbance at 400 nm and increase in absorbance at 290 nm at an interval of 1 nm.
5
Quantifying Total Phenolics in ZH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total phenolic compounds in the ZH sample were measured using the Folin-Ciocalteu protocol [21 (link)]. A total of 1000 μL of ZH was added to 5 mL of Folin-Ciocalteu reagent (mixed with distilled water 1:10 v/v) and 4 mL (75 g/L) of sodium carbonate. The samples were vortexed for 15 s then left to stand for 30 min at 40 °C for staining. The absorbance was measured at a wavelength of 765 nm using the Thermo Scientific Spectrascan UV 2700 dual-beam spectrophotometer. The total phenolic content of ZH was expressed as mg/g n-propyl gallate equivalent (mg GAE/g).
6
Characterization of Silver Nanoparticle-Chitosan Composite Spheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The average diameter of the spheres, expressed as mean ± standard deviation, was obtained from the photographs taken by random sampling of approximately 50 individual particles to minimize selection bias. X-ray diffraction (XRD, D8 Advance, PANalytical X’PERT PRO) patterns were obtained at room temperature using Cu K-α radiation (λ=1.5406 Å) with a range of 2θ=20° to 80°, and a scanning rate of 0.05 s−1. The FTIR spectra were recorded with a Spectrum RXI FTIR Spectrometer, using KBr pellets, in the range of 400 to 4,000 cm−1, with a resolution of 4 cm−1. The micromorphology of the silver nanoparticles–chitosan composite spheres was analyzed using a SEM (S-2700, Hitachi Ltd., Tolyo, Japan) equipped with an energy dispersive spectrometer. The characterization of the silver nanoparticles was carried out using TEM (FEI Tecnai G2 20 S-Twin) and a UV-Vis absorbance spectrophotometer (Thermo Scientific Spectrascan UV 2700). The silver nanoparticles solution was prepared by adding 20 μL CH3COOH solution and 1 mL dd-H2O to ten silver nanoparticles–chitosan composite spheres, and then vortexing for 3– 5 minutes. The silver nanoparticles–chitosan solution was dropped to grid by a micropipette for TEM analysis report.
7
Phenylalanine Ammonia Lyase Extraction and Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PAL was extracted from 0.5 g of P. kurroa shoots, both untreated and treated with optimum concentrations of CA, CAT and CA+CAT, by homogenization in a prechilled mortar and pestle using liquid nitrogen with 5 mL of extraction buffer (100 mM sodium borate buffer, pH 8.8, containing 5 mM β-mercaptoethanol). The homogenate was then centrifuged at 10,000 × g for 20 min. The clarified extract thus obtained was referred to as crude extract and used for enzyme assay. The extraction was performed in triplicates.
The standard assay mixture for PAL contained 100 mM sodium borate buffer (pH 8.8), 15 mM phenylalanine (Phe) and 50 μL crude extract in a total volume of 3.0 mL. The mixture without Phe was preincubated at 30 °C for 20 min to allow for an initial non-enzymatic decrease in absorbance and then the reaction was initiated by the addition of Phe. The increase in absorbance was recorded over a period of 60 min at 290 nm wavelength using a UV-VIS spectrophotometer (SPECTRASCAN UV 2700, Thermo Scientific). The PAL activity was determined by standard curve prepared by taking different concentrations of CA. The activity of PAL was expressed as μmol of CA formed/min/mL under the specified conditions and data has been reported as milliunits (mU) of enzyme. The assay was performed in triplicates.
The standard assay mixture for PAL contained 100 mM sodium borate buffer (pH 8.8), 15 mM phenylalanine (Phe) and 50 μL crude extract in a total volume of 3.0 mL. The mixture without Phe was preincubated at 30 °C for 20 min to allow for an initial non-enzymatic decrease in absorbance and then the reaction was initiated by the addition of Phe. The increase in absorbance was recorded over a period of 60 min at 290 nm wavelength using a UV-VIS spectrophotometer (SPECTRASCAN UV 2700, Thermo Scientific). The PAL activity was determined by standard curve prepared by taking different concentrations of CA. The activity of PAL was expressed as μmol of CA formed/min/mL under the specified conditions and data has been reported as milliunits (mU) of enzyme. The assay was performed in triplicates.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!