Primary hepatocytes were incubated for 24 h in DMEM/F12/10% FBS medium. After attachment, the cells were cultured in serum-free DMEM/F12 for 24 h and then treated with 20 mM CCl4 (in 0.1% DMSO) for 48 h in the presence or absence of EA for the last 24 h. For assess for cell viability, the 10 μl CCK8 reagents (#C0042, Beyotime Institute of Biotechnology) were added to cells and incubated at 37 °C in 5% CO2 for 4 h, and then the plates were measured at 450 nm using the Tecan Safire Multi-detection Microplate Reader (Morrisville, NC, USA).
Multi detection microplate reader
Manufactured by Tecan
Sourced in United States, Switzerland
The Multi-Detection Microplate Reader is a versatile lab equipment designed for various analytical and detection applications. It can measure multiple parameters in microplates, including absorbance, fluorescence, and luminescence. The reader provides accurate and reliable data to support research and development activities.
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Lab products found in correlation
6 protocols using multi detection microplate reader
1
Hepatocyte Viability Assay with CCl4
2
Cytotoxicity Evaluation of Peptide EKL1C
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Cytotoxicity of the peptide EKL1C to the cells (Huh-7, 293T/ACE2, Caco-2) was performed as previously described11 (link). Cells were seeded in a 96-well plate (2 × 104 cells/well) overnight, and EKL1C at graded concentrations was added. After incubation at 37 °C for 48 h, Cell Counting Kit-8 (CCK-8; Dojindo, Kumamoto, Japan) solution was added to measure cell viability, followed by an additional incubation for 4 h. The absorbance was measured at 450 nm with the Multi-Detection Microplate Reader (Tecan, Männedorf, Switzerland). The 50% cytotoxic concentration (CC50) of EKL1C was determined by GraphPad Prism 8.0 software.
3
Microbial Growth Inhibition Assay
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The adjusted microbial inoculum (100 μl) was added to each well of a sterile 96-well flat-bottomed microtiter plate containing the serial dilutions of the tested samples (100 μl/well). Consequently, an inoculum concentration of 5 × 10 5 CFU/ml was obtained in each well. Three wells containing microbial suspensions with no sample using only DMSO employed for dissolving the tested samples (growth control) and two wells containing only media (background control) were included in this plate. Ampicillin, gentamicin and amphotericin B were used as positive controls for Gram-positive, Gram-negative bacteria and fungi, respectively. The tested samples as well as the used standards were used in the concentration range of 460-5000 μg/ml. Optical densities were measured at 600 nm after 24 h at 37 °C for bacteria and after 48 h at 28 °C for fungi using a multi-detection microplate reader (Sunrise-Tecan, Durham, North Carolina, USA). The percentage of growth at each sample concentration was calculated using the following equation: % growth = [(OD 600 of wells containing the test sample/OD 600 of the sample-free well) × 100] after subtraction of background ODs (ODs of microorganism-free wells). Data were measured in triplicates (n = 3) and expressed as means ± S.D.
4
IBSP Serum Concentration Measurement
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The concentrations of IBSP in serums were measured with ELISA Kit (Meimian Biotechnology Co. Ltd) according to the manufacturer's instructions. Data were read by a multi‐detection microplate reader (Tecan) at 450 nm.
5
UCAR-T Cell Cytotoxicity Assay
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MM.1S-mCherry.ffLuc and RPMI-8226-mCherry.ffLuc cells were co-cultured with UCAR-T cells at different E:T ratios (4:1, 2:1, 1:1, and 1:2) in RPMI-1640 medium for 24 h at 37℃. Subsequently, luminescence was measured using a Multi-Detection Microplate Reader (Tecan). In addition, primary MM cells were co-cultured respectively with UCAR-T cells for 24 h at 37℃. Moreover, the levels of IFN-γ, TNF-α, and IL-2 in the harvested supernatants were assessed according to the manufacturer’s instructions (IFN-γ cat# KHC4021, ThermoFisher; TNF-α cat# KAC1751, ThermoFisher; IL-2 cat# BMS221-2, ThermoFisher).
6
Oridonin Cytotoxicity Assessment in DLD-1 Cells
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A CCK-8 kit was used to assess the cytotoxicity of oridonin on DLD-1 cells, following the supplier’s instructions. Cells (5 × 103/well) at the logarithmic phase were cultured in 96-well culture plates overnight. Then, the cells were treated with indicated dosages of oridonin (0, 5, 10, 15, 20 and 25 μM) for 24, 48 and 72 h, respectively. Then, 10 µL of CCK working solution was added into each well. The plates were incubated for 1–3 h under standard conditions. Absorbance at 450 nm was determined using a Multi-Detection Microplate Reader (TECAN, Switzerland).
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