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Dcaf1

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DCAF1 is a laboratory product that functions as an E3 ubiquitin ligase adapter. It is involved in the ubiquitination of target proteins, which can lead to their subsequent degradation. The core function of DCAF1 is to facilitate the transfer of ubiquitin to specific substrate proteins.

Automatically generated - may contain errors

Lab products found in correlation

Flag tag (flag), by Merck Group (3 mentions) Mphosph8, by Proteintech (2 mentions) Pphln1, by Merck Group (2 mentions) Morc2, by Fortis Life Sciences (2 mentions) Setdb1, by Proteintech (2 mentions) Flag tag (flag), by Novus Biologicals (2 mentions) Line 1 orf1p, by Merck Group (2 mentions) Vinculin, by Merck Group (1 mentions) Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by BioLegend (1 mentions)

Spelling variants of this product

Anti-DCAF1 (2 citations)

3 protocols using dcaf1

1

Western Blot Analysis of Protein Markers

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Cells were washed in PBS, counted, normalized for cell number, and lysed directly in 1x SDS-PAGE sample buffer. Samples were run on NuPage 4–12% Bis-Tris gels followed by blotting onto nitrocellulose membranes. Primary antibodies used: FAM208A (Atlas, HPA00875), MPHOSPH8 (Proteintech, 16796–1-AP), PPHLN1 (Sigma, HPA038902), SETDB1 (Proteintech 11231–1-AP), DCAF1 (Proteintech, 11612–1-AP), FLAG (Novus, NB600–345), FLAG (Sigma, F1804, used for IP), MORC2 (Bethyl Labs, A300–149A), LINE-1 ORF1p (Millipore, MABC1152),and HA (Biolegend, 901501).
Yurkovetskiy L., Guney M.H., Kim K., Goh S.L., McCauley S., Dauphin A., Diehl W, & Luban J. (2018). Primate immunodeficiency virus Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Nature microbiology, 3(12), 1354-1361.
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2

Immunoblot and Flow Cytometry Protocols

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Antibodies to Vinculin (cat# V9131, Millipore Sigma), DCAF1 (cat# 11612–1AP, Proteintech), PU.1 (cat# 2266S, Cell Signalling Technology), FLAG (cat# F1804, Millipore Sigma), TET2 (cat#MABE462, EMD Millipore), CAp24 (clone KC57-PE cat# 6604667, Beckman Coulter), Vpr (AIDS Reagent Program cat# 3951 from Dr. Jeffrey Kopp), GFP (cat# ab13970, Abcam) were used for immunoblot analysis. Secondary HRP conjugated antibodies against murine (rat anti-mouse IgG1, eBioscience) and rabbit (goat anti-rabbit IgG, Invitrogen) were also used. Antibodies to PU.1 (clone 7C6B05, BioLegend), FLAG (cat# 637324, BioLegend) were used for flow cytometry.
Virgilio M.C., Disbennett W.M., Chen T., Lubow J., Welch J.D, & Collins K.L. (2023). HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages. bioRxiv.
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3

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in PBS, counted, normalized for cell number, and lysed directly in 1x SDS-PAGE sample buffer. Samples were run on NuPage 4–12% Bis-Tris gels followed by blotting onto nitrocellulose membranes. Primary antibodies used: FAM208A (Atlas, HPA00875), MPHOSPH8 (Proteintech, 16796–1-AP), PPHLN1 (Sigma, HPA038902), SETDB1 (Proteintech 11231–1-AP), DCAF1 (Proteintech, 11612–1-AP), FLAG (Novus, NB600–345), FLAG (Sigma, F1804, used for IP), MORC2 (Bethyl Labs, A300–149A), LINE-1 ORF1p (Millipore, MABC1152),and HA (Biolegend, 901501).
Yurkovetskiy L., Guney M.H., Kim K., Goh S.L., McCauley S., Dauphin A., Diehl W, & Luban J. (2018). Primate immunodeficiency virus Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Nature microbiology, 3(12), 1354-1361.
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