Whole cell lysates were prepared and loaded onto SDS-PAGE. After transfer, the membrane was blocked with 5% skim milk for 1hr and then incubated in diluted primary antibodiesATP11B (ab05377), ATP7B (ab124973), tubulin (ab210797) or GAPDH (ab181602), obtained from Abcam, Cambridge, USA) overnight. The membrane was washed with TBS-T three times and incubated with an HRP-labeled secondary antibody for 1hr. The membrane was developed using a Bio-Rad developer system.
Developer system
Manufactured by Bio-Rad
The Developer system is a laboratory equipment designed for the development and processing of various types of film or membrane samples. It provides a controlled environment for the automated development, fixing, washing, and drying of these materials. The core function of the Developer system is to facilitate the processing of film or membrane samples in a standardized and consistent manner.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using developer system
1
Western Blot Protein Analysis
2
Western Blot Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole cell lysates were prepared and loaded onto sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). After transfer, the membrane was blocked with 5% skim milk in Tris-buffered saline (TBS) supplemented with 0.05% Tween 20 (TBST) for 1 hour and then incubated in diluted primary antibodies PKR, p90RSK, tubulin (#ab210797) or GAPDH (#ab181602) (obtained from Abcam, Cambridge, USA) overnight. The membrane was washed with TBS-T three times and incubated with an horseradish peroxidase (HRP)-labeled secondary antibody for 1 hour. The membrane was developed using a Bio-Rad developer system.
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