Ftir 4000 instrument

The FTIR spectra were obtained by means of a FTIR-4000 instrument (JASCO, Tokyo, Japan) and peaks were given in terms of wave number (cm⁻¹). The ¹H-NMR and ¹³C-NMR spectra of the synthesized compounds were recorded on an Avance II 400 NMR spectrometer (Bruker, Biospin AG Industriestrasse 26, CH-8117, Fallanden, Switzerland)at 400/100 MHz frequency in CDCl₃ and using TMS as internal standard (chemical shift δ in ppm). The ³¹P-NMR spectra of compounds were recorded in CDCl₃ using phosphoric acid (H₃PO₄) as external standard (chemical shift δ in ppm). The mass spectra were executed on a Micromass Q-Tof system (Waters, UK). Elemental analyses were done with a FLASHEA 112 analyzer (Shimadzu, Mumbai, Maharashtra, India) and all analyses were consistent (within 0.4%) with theoretical values. A Vibra Cell VCX-500 ultrasound synthesizer (Sonics, Newtown, CT, USA) equipped with a solid probe was employed for the synthesis of intermediate 1. In vitro anti-cancer activity screening of the synthesized compounds was accomplished at the Anti-Cancer Drug screening facility (ACDSF) at ACTREC (Tata Memorial Centre, Navi Mumbai, India).

All the reactions were performed in oven-dried glassware. All the reagents and solvents were used as obtained from the supplier or recrystallized/redistilled unless otherwise noted. The purity of the synthesized compounds was monitored by ascending thin layer chromatography (TLC) on silica gel-G coated aluminum plates (Merck, Darmstadt, Germany), visualized by iodine vapor. Melting points were determined in open capillary tubes. The FTIR spectra were obtained using a FTIR-4000instrument (JASCO, Tokyo, Japan) and peaks were expressed in terms of wave- number (cm⁻¹). The ¹H-NMR and ¹³C-NMR spectra of the synthesized compounds were recorded in CDCl₃ at 400/100 MHz on an Avance 400 NMR Spectrometer II (Bruker, Biospin AG Industriestrasse 26, CH-8117, Fallanden, Switzerland) and using TMS as internal standard (chemical shift δ in ppm), Mass spectra were scanned on a Micromass Q-Tof system (Waters, UK). Elemental analyses (C, H and N) were done with a FLASHEA 112 analyzer (Shimadzu, Mumbai, Maharashtra, India) and all the analyses were consistent (within 0.4%) with the theoretical values.