L thyroxine sodium salt pentahydrate t4

L-Thyroxine sodium salt pentahydrate (T4, Sigma Chemical Co., St. Louis, MO, USA) dissolved in saline vehicle (154 nM NaCl) was injected i.p. twice at 1 and 6 h after FPI in the treatment group (n = 6 mice) at 1.2 µg/100 g body weight. Control FPI mice (n = 6) received vehicle (saline).

Oli-neu cells were maintained in DMEM, high glucose GlutaMAX^TM Supplement, pyruvate (Gibco reference 31966047, Thermo Fisher Scientific, Courtaboeuf, France) supplemented with 1% N2 (Gibco reference 17502001, Thermo Fisher Scientific, Courtaboeuf, France), 1% heat inactivated horse serum (Gibco reference 26050070, Thermo Fisher Scientific, Courtaboeuf, France), 1% penicillin-streptomycin (10,000 U/mL) (Gibco reference 15140122, Thermo Fisher Scientific, Courtaboeuf, France), 10 µg/mL insulin from bovine pancreas (Sigma I5500, Saint Quentin Fallavier, France), 0.4 µg/mL L-Thyroxine sodium salt pentahydrate (T4) (Sigma T0397, Saint Quentin Fallavier, France,), and 0.34 µg/mL 3,3′,5-Triiodo-L-thyronine sodium salt (T3) (Sigma T6397 Saint Quentin Fallavier, France).
Flasks and wells were coated with poly-L-lysine (Sigma P4707, Saint Quentin Fallavier, France) diluted at 20 µg/mL. Cells were exposed to BPA (Sigma 239658, Saint Quentin Fallavier, France) during 72 h of proliferation and an additional 72 h of differentiation induced by 1 μM PD174265 in DMSO (Sigma 513040-M, Saint Quentin Fallavier, France). The medium was changed after 48 h of differentiation. Oli-neu cells were exposed to 0.01, 0.1, 1, 10, 20, 50, and 100 µM BPA in DMSO. The final concentration of DMSO was 0.2%.