Cytotoxicity detection kit assays

Propofol (2,6-diisopropylphenol) was purchased from Sigma-Aldrich (St. Louis, MO, USA). An antibody against DENV NS1 (Cat# GTX124280) was purchased from GeneTex (San Antonio, TX); antibodies against phospho-STAT1^Tyr701 (Cat# 9167; clone 58D6), STAT1 (Cat# 9172), horseradish peroxidase- (HRP-) conjugated goat anti-rabbit IgG (Cat# 7074S), and HRP-conjugated horse anti-mouse IgG (Cat# 7076S) were purchased from Cell Signaling Technology (Beverly, MA); Alexa Flour 488-conjugated goat anti-mouse antibody (Cat# A-11029) and Hoechst 33258 (Cat# H3569) were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA); antibody against dsRNA (Cat# 10010200) was purchased from SCICONS; antibody against mouse β-actin (Cat# A5441), 4,6-diamidino-2-phenylindole (DAPI; Cat# D9542), the V-ATPase inhibitor bafilomycin A1 (Baf A1; Cat# 19-148), and acridine orange hemi (zinc chloride) salt (Cat# A6014) were purchased from Sigma-Aldrich (St. Louis, MO). According to the manufacturer's instructions, cell cytotoxicity was assessed using Cytotoxicity Detection Kit assays (Roche Diagnostics, Lewes, UK).

For the detection of cell viability and cytotoxicity, a colorimetric Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan) and Cytotoxicity Detection kit assays (Roche Diagnostics, Lewes, UK) were respectively assessed according to the manufacturer's instructions. The concentration of formazan WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2Htetrazolium,monosodium salt] and lactate dehydrogenase in each sample is then directly determined from the increase in absorbance at 460 and 340 nm, respectively, by using a microplate reader (SpectraMax 340PC; Molecular Devices Corporation, Sunnyvale, CA, USA).

Cell viability and cytotoxicity were respectively assessed using a colorimetric Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan) and Cytotoxicity Detection kit assays (Roche Diagnostics, Lewes, UK), according to the manufacturer’s instructions.