Lactobacillus de Man, Rogosa, and Sharpe (MRS) agar and broth, oxgall salt glycerol, phenol, NaCl, xylene, deoxyribonuclease (DNase) agar medium, blood agar medium with 5% (w/v) sheep blood, ABTS, DPPH, and, antibiotic susceptibility disc were procured from HiMedia Laboratories Pvt. Ltd., Mumbai, India. The pathogens namely, Bacillus subtilis MTCC 10403, Escherichia coli MTCC 4430, Pseudomonas aeruginosa MTCC 424, Micrococcus luteus MTCC 1809, and Salmonella typhimurium MTCC 98 were procured from the Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh, India.
Blood agar medium
Manufactured by HiMedia
Sourced in India
Blood agar medium is a type of microbiological culture medium used for the isolation and identification of bacteria. It consists of a base agar medium supplemented with defibrinated sheep or horse blood. The blood agar medium supports the growth of a wide range of bacteria and provides information about their hemolytic properties.
Automatically generated - may contain errors
Lab products found in correlation
Co2 incubator, by Thermo Fisher Scientific (3 mentions)
Antibiotic susceptibility disc, by HiMedia (2 mentions)
Deoxyribonuclease dnase agar medium, by HiMedia (2 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by HiMedia (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by HiMedia (2 mentions)
Bacillus subtilis mtcc 10403, by Microbial Type Culture Collection (2 mentions)
Escherichia coli mtcc 4430, by Microbial Type Culture Collection (2 mentions)
Pseudomonas aeruginosa mtcc 424, by Microbial Type Culture Collection (2 mentions)
Micrococcus luteus mtcc 1809, by Microbial Type Culture Collection (2 mentions)
Salmonella typhimurium mtcc 98, by Microbial Type Culture Collection (2 mentions)
Multiskan fc, by Thermo Fisher Scientific (2 mentions)
Phenol, by HiMedia (1 mentions)
Xylene, by HiMedia (1 mentions)
Sheep blood, by HiMedia (1 mentions)
Sodium chloride (nacl), by HiMedia (1 mentions)
Macconkey agar, by HiMedia (1 mentions)
Triple sugar iron agar, by HiMedia (1 mentions)
Egg yolk agar medium, by HiMedia (1 mentions)
Phosphate buffer saline (pbs), by HiMedia (1 mentions)
Starch agar medium, by HiMedia (1 mentions)
4 protocols using blood agar medium
1
Antimicrobial Activity Assessment of Lactobacillus
2
Antimicrobial Potential of Lactobacillus
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Lactobacillus de Man, Rogosa, and Sharpe (MRS) agar and broth, glycerol, NaCl, oxgall salt, xylene, phenol, blood agar medium with 5% (w/v) sheep blood, antibiotic susceptibility disc, ABTS, DPPH, and deoxyribonuclease (DNase) agar medium required for this study were obtained from HiMedia Laboratories Pvt. Ltd., Mumbai, India. The pathogens (Escherichia coli MTCC 4430, Bacillus subtilis MTCC 10403, Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424, and Salmonella typhimurium MTCC 98) were obtained from Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh, India. Instruments used were microplate reader Multiskan FC, CO2 incubator (Thermo Fisher Scientific, Mumbai, India). The centrifugation unit used in this study was the REMI refrigerated micro centrifuge (RPM 7000, C-30 Plus; relative centrifugal force: 20,000× g to 22,000× g).
3
Collection and Identification of A. baumannii
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A. baumannii were collected from clinical samples, over a six-month period (December 2021 to June 2022). The specimens included respiratory, blood, wounds, burns, surgery, urine, and cerebrospinal fluid (CSF) samples obtained from hospitalized patients in three hospitals in the province of Isfahan (Al-Zahra, Amin, and Milad). These samples were cultured on MacConkey agar and blood agar medium (HiMedia, India) and then were incubated for 24 hours at 37°C. Gram staining and biochemical testing were used to identify the pure strains [17 (link)].
4
Soil Microbial Diversity in Nepal
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This study was carried out in Central Campus of Technology Hattisar Dharan and Regional Agricultural Research Station (RARS) Tarhara from October 2018 to March 2019. Soil samples were collected from different altitudes ranging from Itahari (374 ft.), Tarhara (418 ft.), Dharan (1272 ft.) and Vedetar (5140 ft.) of Koshi Zone, Nepal. From each geographical region 25 different soil samples were collected and in total 100 different soil samples from four regions were collected. Soil samples weighing 10gm was collected in sterile plastic bags from 3-5cm depth and transported into the laboratory at 4°C. Materials used in this experiment were Sodium acetate (HiMedia, India), Lauria Bertani (LB) broth (HiMedia, India). Nutrient Agar (HiMedia, India), Coomassie brilliant blue (HiMedia, India), Phosphate buffer saline (HiMedia, India), Grams staining reagents (HiMedia, India), MR-VP medium and reagents (HiMedia, India), Indole medium (HiMedia, India), Citrate agar medium (HiMedia, India), Gelatin agar medium (HiMedia, India), Starch agar medium (HiMedia, India), Egg yolk agar medium (HiMedia, India), Blood agar medium (HiMedia, India), Carbohydrate fermentation broth (HiMedia, India), SIM medium (HiMedia, India), Triple sugar iron agar (HiMedia, India).
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