Master hpi t plus

Arabidopsis thaliana accession Col-0 was used as WT. The following mutants were used: ahk2-5 ahk3-7 (Riefler et al., 2006 (link)), cca1-1 lhy-20 (Nitschke et al., 2016 (link)). Arabidopsis plants were grown on soil for 5 weeks under short day (SD) conditions (8 h light/16 h darkness) in a growth chamber with light intensities of 100–150 μmol m^–2 s^–1, using a combination of Philips Son-T Agros 400 W and Philips Master HPI-T Plus, 400 W/645 lamps, at 22°C and 60% relative humidity.

Potassium tert-butoxide, 4-methoxybenzenethiol (3), benzenethiol (4), naphthalene-2-thiol (11) and potassium diphenylphosphide solution (0.5 M in THF) are commercially available and used as received. Methyl-4-iodocubane-1-carboxylate (1) and 1,4-diiodocubane (2) were synthesized according to ref. 18 (link). DMSO is Carlo Erba and stored under molecular sieves (4 Å). ¹H NMR and ¹³C NMR spectra were recorded on a 400 MHz Bruker nuclear magnetic resonance spectrometer. HR-MS were recorded on a Bruker, MicroTOF Q II equipment, operated with an ESI source in (positive/negative) mode, using nitrogen as nebulizing and drying gas and sodium formate 10 mM as internal standard. Gas chromatographic analyses were performed on a Varian 3900 GC with flame ionization detector on a FactorFour capillary column (VF-5 MS, 30 m, 0.32 mm, 0.25 micron). GC-MS analyses were carried out on a Shimadzu GC-MS QP5050 spectrometer, employing a 30 m, 0.32 mm, 0.25 micron, DB-5 MS column. Irradiation was performed in a reactor equipped with two 400 W lamps (Philips model Master HPI-T Plus, air- and water-cooled). The Fig. SI-1 in ESI† shows the spectrum of the lamps. HPLC analyses were carried out on a Waters 1525 Binary HPLC Pump connected to a Waters 2998 Photodiode Array Detector, and employing an Agilent Zorbax Eclipse XDB-C18 Analytical column (4.6 × 150 mm, 5 μm).

All samples were generally treated in the same way. Liquid samples were transferred to mineral medium [22 (link)] on site. In case of a short travel distance, the samples were brought to the lab immediately, in other cases samples were stored at windows in hotel rooms until transport to the lab. The cultures were incubated in Erlenmeyer flasks at 21 °C, in elevated CO₂ concentration (approx. 1.5 vol%), at 85–95 rpm (orbital shaker VKS 75 control, Edmund Bühler GmbH, Bodelshausen, Germany) and a day–night cycle of 16:8 h with an illumination of approx. 108 μmol photons m⁻² s⁻¹ (metal halide lamp, Philips Master HPI-T Plus, 250 W) until the first green coloration visible to the bare eye. After that, cultures were inoculated into fresh BG-11 medium approx. every three to six days to pre-select the fastest growing photo-autotrophs.
In addition to the isolated wild type strains, screening and methods for obtaining axenic cultures were also carried out with the strains Synechocystis sp. PCC6803 and Synechocystis cf. salina CCALA192 as reference, which were obtained from the Pasteur culture collection (FRA) and from the culture collection of autotrophic organisms (CZE), respectively, both in non-axenic form.