Anti kmt2d antibody

Immunofluorescence and immunoblotting were performed as previously described [19 (link)]. The following antibodies were used for immunoblotting. Anti-KMT2D antibody (dilution 1:1000, #ABE1867, Sigma); anti-CD133 antibody (dilution 1:1000, #18,470, Proteintech); anti-ALDH1A1 antibody (dilution 1:2000, #15,910, Proteintech); anti-OCT4 antibody (dilution 1:1000, #11,263, Proteintech); anti-β-catenin antibody (dilution 1:2000, #51,067, Proteintech); anti-c-Myc antibody (dilution 1:100, #ab32072, Abcam); anti-Cyclin D1 antibody (dilution 1:1000, #26,939, Proteintech); anti-H3K4me1 antibody (dilution 1:500, #ab8895, Abcam); and anti-H3K27ac antibody (dilution 1:500, #ab4729, Abcam); anti-MEF2A antibody (dilution 1:500, #12,382, Proteintech). The following antibodies were used for immunofluorescence. Anti-KMT2D: antibody (dilution 1:100, #27,266, Proteintech); anti-CD133 antibody (dilution 1:200, #18,470, Proteintech); anti-β-catenin antibody (dilution 1:200, #51,067, Proteintech).

Immunohistochemistry studies were conducted using the following antibodies. Anti-KMT2D antibody (dilution 1:100, #27,266, Proteintech); anti-β-catenin antibody (dilution 1:2000, #51,067, Proteintech); anti-CD133 antibody (dilution 1:1000, #18,470, Proteintech); anti-c-Myc antibody (dilution 1:100, ab32072, Abcam). Histologic sections on human tissue microarrays were cut at 5 μm. The immunochemical test kit was from MaiXin Ltd. (Fu Zhou, China). Peroxidase blocker and 5% goat serum were added in sequence to histologic sections, after which the primary antibody was incubated overnight at 4 °C. The corresponding secondary antibody and avidin–biotin-peroxidase were then added, and DAB and hematoxylin were employed for visualization. We used Aperio ImageScope software (Version 9.1; Leica, Wetzlar, Germany) for analysis.