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Mouse monoclonal anti human glucagon

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Mouse monoclonal anti-human glucagon is an antibody that recognizes human glucagon. Glucagon is a hormone produced by the pancreas that plays a role in regulating blood glucose levels.

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Lab products found in correlation

Polyclonal goat anti somatostatin, by Santa Cruz Biotechnology (7 mentions) Polyclonal guinea pig anti porcine insulin, by Agilent Technologies (7 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (6 mentions) 649 conjugated secondary antibodies, by Jackson ImmunoResearch (5 mentions) Dylight 488, by Jackson ImmunoResearch (5 mentions) Monoclonal mouse anti human cd45, by Agilent Technologies (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Rat anti mouse cd4 and cd8, by BD (1 mentions)

Spelling variants of this product

Glucagon (128 citations) Mouse anti-glucagon (49 citations) Anti-glucagon (20 citations) Human glucagon (2 citations) Monoclonal mouse (2 citations)

7 protocols using mouse monoclonal anti human glucagon

1

Pancreatic Tissue Sectioning and Immunostaining

Cited 1 time
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The pancreas was divided into consecutive tissue blocks (~5 mm) with alternating collection between paraffin-embedding and snap frozen18 (link). In this study, paraffin-embedded sections (5 μm) were stained with the following primary antibodies (all 1:500): polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich, St. Louis, MO), polyclonal goat anti-somatostatin (Santa Cruz, Santa Cruz, CA), polyclonal goat anti-human PP (DAKO), and DAPI (Invitrogen, Carlsbad, CA). The primary antibodies were detected using a combination of DyLight 488, 549, and 649-conjugated secondary antibodies (1:200, Jackson ImmunoResearch Laboratory, West Grove, PA).
Poudel A., Fowler J.L., Zielinski M.C., Kilimnik G, & Hara M. (2016). Stereological analyses of the whole human pancreas. Scientific Reports, 6, 34049.
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2

Multimodal Immunohistochemistry Analysis

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Paraffin-embedded sections (5 μm) were stained with the following primary antibodies (all 1:500): polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich, St. Louis, MO), polyclonal goat anti-somatostatin (Santa Cruz, Santa Cruz, CA), monoclonal mouse anti-human CD45 (DAKO), and DAPI (Invitrogen, Carlsbad, CA). The primary antibodies were detected using a combination of DyLight 488, 549, and 649-conjugated secondary antibodies (1:200, Jackson ImmunoResearch Laboratory, West Grove, PA).
Poudel A., Savari O., Striegel D.A., Periwal V., Taxy J., Millis J.M., Witkowski P., Atkinson M.A, & Hara M. (2015). Beta-cell destruction and preservation in childhood and adult onset type 1 diabetes. Endocrine, 49(3), 693-702.
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3

Immunostaining of Isolated Islets

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Isolated islets were stained with the following primary antibodies (all 1∶500): polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich, St. Louis, MO), polyclonal goat anti-somatostatin (Santa Cruz, Santa Cruz, CA), and DAPI (Invitrogen, Carlsbad, CA). The primary antibodies were detected using a combination of DyLight 488, 549, and 649-conjugated secondary antibodies (1∶200, Jackson ImmunoResearch Laboratory, West Grove, PA).
Hoang D.T., Matsunari H., Nagaya M., Nagashima H., Millis J.M., Witkowski P., Periwal V., Hara M, & Jo J. (2014). A Conserved Rule for Pancreatic Islet Organization. PLoS ONE, 9(10), e110384.
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4

Histological Analysis of Mouse Pancreas

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Mouse pancreata were excised and frozen or paraffin-embedded. Sections were stained with hematoxylin and eosin (H&E), and immunostained with the following primary antibodies (all 1:500): rat anti-mouse CD4 and CD8 (BD Biosciences Pharmingen, San Diego, CA), polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich, St. Louis, MO) and polyclonal goat anti-somatostatin (Santa Cruz, Santa Cruz, CA). Nuclei were stained with DAPI (Sigma-Aldrich). The primary antibodies were detected using ABC staining (Thermo Fisher Scientific, Rockford, IL) or a combination of DyLight 488, 549, and 649-conjugated secondary antibodies (1:200, Jackson ImmunoResearch Laboratory, West Grove, PA).
Grapov D., Fahrmann J., Hwang J., Poudel A., Jo J., Periwal V., Fiehn O, & Hara M. (2015). Diabetes Associated Metabolomic Perturbations in NOD Mice. Metabolomics : Official journal of the Metabolomic Society, 11(2), 425-437.
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5

Immunohistochemical Analysis of Pancreatic Tissue

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At the time of necropsy, pancreata were dissected and fixed in 4% paraformaldehyde overnight and paraffin-embedded. Tissue sections (5 μm in thickness) were immunostained with the following primary antibodies (all at 1:500 dilution): polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich, St. Louis, MO), polyclonal goat anti-somatostatin (Santa Cruz, Santa Cruz, CA), and DAPI (Invitrogen, Carlsbad, CA). The primary antibodies were detected using a combination of DyLight 488, 549, and 649-conjugated secondary antibodies (1:200, Jackson Immuno Research Laboratory, West Grove, PA).
Balise V.D., Cornelius-Green J.N., Parmenter B., Baxter S., Kassotis C.D., Rector R.S., Thyfault J.P., Paterlini S., Palanza P., Ruiz D., Sargis R, & Nagel S.C. (2019). Developmental Exposure to a Mixture of Unconventional Oil and Gas Chemicals Increased Risk-Taking Behavior, Activity and Energy Expenditure in Aged Female Mice After a Metabolic Challenge. Frontiers in Endocrinology, 10, 460.
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6

Immunohistochemical Analysis of Pancreas

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At the time of tissue harvest, the pancreas was dissected, weighed, and fixed in 4% paraformaldehyde overnight followed by paraffin embedding. Pancreas sections (5 μm in thickness) were immunostained with the following primary antibodies at 1:500 dilution: polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich), polyclonal goat anti-somatostatin (Santa Cruz Biotechnology, Santa Cruz, CA), and DAPI (Invitrogen, Carlsbad, CA). The primary antibodies were detected using a combination of DyLight 488-, 549-, and 649-conjugated secondary antibodies (1:200, Jackson Immuno Research Laboratory, West Grove, PA). Antibodies used in this study have been previously validated [36 ].
Ruiz D., Regnier S.M., Kirkley A.G., Hara M., Haro F., Aldirawi H., Dybala M.P, & Sargis R.M. (2019). Developmental Exposure to the Endocrine Disruptor Tolylfluanid Induces Sex-Specific Later-Life Metabolic Dysfunction. Reproductive toxicology (Elmsford, N.Y.), 89, 74-82.
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7

Immunohistochemical Analysis of Pancreatic Islets

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The pancreas was divided into consecutive tissue blocks (~5 mm) with alternating collection between paraffin-embedding and snap frozen18. In this study, paraffin-embedded sections (5 μm) were stained with the following primary antibodies (all 1:500): polyclonal guinea pig anti-porcine insulin (DAKO, Carpinteria, CA), mouse monoclonal anti-human glucagon (Sigma-Aldrich, St. Louis, MO), polyclonal goat anti-somatostatin (Santa Cruz, Santa Cruz, CA), and DAPI (Invitrogen, Carlsbad, CA). The primary antibodies were detected using a combination of DyLight 488, 549 and 649-conjugated secondary antibodies (1:200, Jackson ImmunoResearch Laboratory, West Grove, PA).
Olehnik S.K., Fowler J.L., Avramovich G, & Hara M. (2017). Quantitative analysis of intra- and inter-individual variability of human beta-cell mass. Scientific Reports, 7, 16398.
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