The measurement of ATP content was analyzed using ATP bioluminescence assay kit (11699709001; Roche Life Science, Indianapolis, IN, USA) according to the manufacturer's instructions. After 2‐h reperfusion, the PRNCs were incubated with cell lysis reagent (Sigma, Fremont, CA, USA) and protease inhibitor cocktail (Sigma) to the samples for 5 min at room temperature in darkness. Luciferase reagent was added to the samples, and the luminescence of the live cells was measured by the Gemini EX florescence plate reader (Molecular Device).
Luciferase reagent
Manufactured by Molecular Devices
The Luciferase reagent is a laboratory product designed to facilitate bioluminescence detection and quantification. It contains the luciferase enzyme, which catalyzes a light-emitting reaction when mixed with a luciferin substrate and ATP. This reagent is used to measure the activity of luciferase-tagged proteins or cells, allowing for sensitive detection and analysis of various biological processes.
Automatically generated - may contain errors
2 protocols using luciferase reagent
1
ATP Content Measurement Protocol
2
ATP Content Measurement in PRNCs
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