Anti p53 clone 1c12

Manufactured by Cell Signaling Technology

Anti-p53 (clone 1C12) is a mouse monoclonal antibody that recognizes the p53 protein. p53 is a tumor suppressor protein that plays a crucial role in regulating cell growth and division. The Anti-p53 (clone 1C12) antibody can be used to detect and study the p53 protein in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd28 37, by BioLegend (1 mentions) Anti cd45.1 clone a20, by BD (1 mentions) Anti α tubulin dm1a, by Thermo Fisher Scientific (1 mentions) Anti cd3ε 145 2c11, by BioLegend (1 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-p53 (256 citations) Anti-p53 (1C12, (13 citations) P53 (clone 1C12; (3 citations) (clone 1C12 (2 citations) Anti-human p53 mouse monoclonal (clone:1C12) (2 citations) Mouse anti-p53 antibody (clone 1C12; (2 citations)

2 protocols using anti p53 clone 1c12

Antibody Panel for T Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used.
For stimulation and cell culture: anti-CD3ε (145-2C11) and anti-CD28 (37.51) antibodies were from BioLegend. For cell sorting and flow cytometry analysis: anti-CD8α (clone 53-6.7), anti-CD4 (clone RM4-5), anti-CD24 (clone M1/69), anti-TCRβ (clone H57-597), anti-Vα11 (clone RR8-1), anti-CD5 (clone 53-7.3), anti-CD69 (clone H1.2F3), anti-B220 (clone RA3-6B2), anti-Gr1 (clone RB6-8C5), anti-CD11b (clone M1/70), anti-CD27 (clone LG.3A10), anti-CD11c (clone N418), anti-Ter119 (clone TER119), anti-CD3 (clone 145-2C11), anti-NK1.1 (clone PK136), anti-TCRγδ (clone GL3), anti-CD44 (clone IM7), anti-CD25 (clone PC61.5), anti-CD71 (clone R17217), anti-IL-7R (clone A7R34), anti-BCL-2 (clone 3F11), anti-CD19 (clone 1D3/CD19), anti-c-kit (clone 2B8), anti-IgM (clone RMM-1), and anti-CD45.1 (clone A20) were from BD Biosciences and BioLegend. For imaging studies: anti-γ-tubulin (clone 14C11) was from BioLegend and anti-α-tubulin (DM1A) was from (Thermo Fisher Scientific). For immunoprecipitation and Western blot analysis: anti-DIC (clone 74-1), IgG2b isotype control (sc-3879), anti-LIS1 (sc-15319), and anti-DHC (sc-9115) were from Santa Cruz Biotechnologies. Anti-p150glued (clone 1/p150Glued) were from BD Biosciences, anti-p53 (clone 1C12) were from Cell Signaling, and anti-Rac1 (clone 23A8) were from Millipore.

Argenty J., Rouquié N., Bories C., Mélique S., Duplan-Eche V., Saoudi A., Fazilleau N, & Lesourne R. (2022). A selective LIS1 requirement for mitotic spindle assembly discriminates distinct T-cell division mechanisms within the T-cell lineage. eLife, 11, e80277.

+ Open protocol

+ Expand

Immunoblotting of p53 in pro-B cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultured pro-B cells or CD3-CD4-CD8-Ter119-depleted thymocytes were lysed in RIPA buffer. 10 ug total protein was run on a 10% acrylamide gel and transferred to PVDF by wet electrophoretic transfer. Membranes were probed with anti-p53 (clone 1C12, Cell Signaling Technologies) and anti-beta actin (product ab8227, Abcam) followed by secondary HRP-conjugated antibodies and detection by ECL according to the manufacturer’s protocol (SuperSignal West Dura Extended Duration Substrate, Thermoscientific).

Jacobsen J.A., Woodard J., Mandel M., Clark M.R., Bartom E.T., Sigvardsson M, & Kee B.L. (2017). EZH2 regulates the developmental timing of effectors of the pre-antigen receptor checkpoints. Journal of immunology (Baltimore, Md. : 1950), 198(12), 4682-4691.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!