Vectastain elite abc rat immunoglobulin g kit

Formalin-fixed, paraffin-embedded, decalcified joints were stained with a monoclonal TK10-79 antibody recognizing mouse VAP-1 or a negative class-matched control antibody (both 1 μg/ml). Antigen retrieval with 0.01 M citric acid (pH 6.0) and removal of endogenous peroxidase with 1% H₂O₂ were performed. The primary antibody was detected with a VECTASTAIN Elite ABC rat immunoglobulin G kit (PK-6104; Vector Laboratories, Burlingame, CA, USA), followed by horseradish peroxidase-conjugated avidin. Diaminobenzidine (K3468; DAKO, Glostrup, Denmark) was used as a chromogen. The samples were counterstained with hematoxylin.

With a written informed consent, tissue samples were collected from the HCC cases who were diagnosed with imaging studies including magnetic resonance imaging and computed tomography, and underwent surgical resection in Niigata University Hospital. The tissues were stained with hematoxylin and eosin or immunohistochemical stains: Rat anti‐Ctip2 (Bcl11b) antibody (ab18465; Abcam), Rabbit anti‐GATA6 antibody (ab175349; Abcam), Vectastain Elite ABC Rat immunoglobulin G kit (PK‐6104; Vector Laboratories), Vectastain Elite ABC Rabbit immunoglobulin G kit (PK‐6101; Vector Laboratories, Burlingame, CA), and 3,3′‐diaminobenzidine chromogen tablets (Muto Pure Chemicals). The expression of BCL11B and GATA6 was confirmed via RT‐PCR in recently resected 70 cases using the aforementioned procedure. The tumor tissues of these cases were immunohistochemically stained to determine the relationship between BCL11B and GATA6. Images were captured randomly, and analyzed quantitatively, using the ImageJ software (version 1.8.0_172; National Institutes of Health, Bethesda, MD).³⁸