Avance av 300

SEC analyses of polymers mCTA2 and 6–10 were performed on a Varian PL-GPC 50 Plus instrument fitted with mixed C columns and an RI detector using 5 mM NH₄BF₄ in N,N-dimethylformamide (DMF) as the eluent. SEC analyses of polymers mCTA1, mCTA3, mCTA4, 1–5 and 11–17 were performed on a Varian PL-GPC 50 Plus instrument fitted with mixed C columns and an RI detector using tetrahydrofuran (THF) containing 2% triethylamine (TEA) as the eluent. M_n values determined by SEC were calculated using poly(methyl methacrylate) standards. Additional triple detection SEC analysis was performed on mCTA1 using 5 mM NH₄BF₄ in DMF as the eluent.
¹H NMR spectroscopy was performed at 300 MHz on a Bruker Avance III HD-300 or a Bruker Avance AV-300 spectrometer, or at 400 MHz a Bruker Avance III HD-400 spectrometer. ¹³C NMR was performed at 75 MHz or 100 MHz on a Bruker Avance AV-300 or a Bruker Avance III HD-400 spectrometer respectively. For ¹H and ¹³C NMR spectroscopy, chemical shifts (δ) in parts per million (ppm) are reported relative to the residual CHCl₃ solvent peak at 7.26 ppm or 77.0 ppm, respectively.
High resolution electrospray ionization time of flight mass spectrometry (HRMS (ESI-ToF)) was performed on a Bruker MaXis mass spectrometer. Fourier transform infra-red (FT-IR) spectroscopy was performed on a Perkin-Elmer Spectrum 100 FT-IR spectrometer.

NAPG was synthesized according to a previous research with some modifications (Meng et al., 2015 (link)). Briefly, NAC (1 mmol) was dissolved in anhydrous N,N-dimethylformamide (5 mL) and then 0.63 mmol EDCI was added. This reaction was continued for 1 h in an ice bath. Afterwards, S100 (0.43 mmol) was dissolved in CH₂Cl₂ (25 mL) and slowly added to the above solution, which was first stirred for 3 h in an ice bath and then stirring was continued for 72 h at room temperature. Subsequently, the organic solvent was removed using a rotary evaporator. The resultant solution was dialyzed with a dialysis bag (3.5 kDa MW cutoff, Sigma-Aldrich, Milwaukee, WI) at room temperature for 48 h to remove the unreacted NAC and lyophilized to obtain NAPG. The chemical structure of NAPG was analyzed by ¹H-NMR spectra (AVANCE AV-300, Bruker Instrument Inc., Switzerland) with D₂O as the solvent at 25 °C. Furthermore, surface tension measurements (Shanghai Shibo. Instrument Co. Ltd., Shanghai, China) were also done to measure the critical micelle concentration (CMC). Briefly, S100-NAPG and S100 solutions (0.001–1.5 mg/mL) were prepared. The surface tension of solutions was measured and plotted against the logarithm of sample concentration; the CMC was determined by taking the cross-point when extrapolating the surface tension.