Prg4 creert2

All animal experiments were conducted in accordance with the guidelines described in the Guide for the Use and Care of Laboratory Animals of the Institute for Laboratory Animal Research (ILAR, 2011). Furthermore, animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of The University of Tokyo (M-P17-091). All animals were housed in a facility under the supervision of the IACUC at 18° to 22°C with a 12-hour light/dark cycle and were allowed free access to food and water. Genotypes were analyzed in each experiment. For all in vitro and in vivo studies requiring WT mice, adult male (8 weeks old) C57BL/6J mice were purchased from Sankyo Lab Services (Tokyo, Japan). The comparative groups were maintained on a C57BL/6J background. TOPGAL mice, Prg4-Cre^ERT2, and Rosa26-tdTomato mice were obtained from the Jackson Laboratory (Sacramento, CA). We generated Prg4-Cre^ERT2;R26-tdTomato mice by mating Prg4-Cre^ERT2 male mice with R26-tdTomato female mice. The primer sequences used for genotyping are shown in table S5. For the generation of CAG-EGFP-Rspo2 mice, the transgene shown in Fig. 5A was constructed. DNA purification and microinjection were performed in accordance with standard protocols.