TYLMS-1 and TC616 cells were seeded in 96-well plates at 4.0 × 103 cells and 2.0 × 103 cells per well, respectively. Four hours after seeding, the cell viability in each well was measured as a standard value using the CellTiter-Glo 2.0 Cell Viability kit (Promega, Madison, WI, USA). The relative cell viability was monitored every 24 h for up to 72 h. The assay was read on an EnSight Multimode Plate Reader (PerkinElmer, Waltham, MA, USA).
Celltiter glo 2.0 cell viability kit
Manufactured by Promega
Sourced in United States
The CellTiter-Glo 2.0 Cell Viability kit is a luminescent assay that quantifies the amount of ATP present in a cell culture as an indicator of metabolically active cells. The assay measures the luminescent signal produced by the luciferase-catalyzed reaction of ATP with the stabilized luciferase substrate.
Automatically generated - may contain errors
3 protocols using celltiter glo 2.0 cell viability kit
1
Cell Viability Monitoring in 96-Well Plates
2
Intracellular ATP Quantification Assay
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Commercially available CellTiter-Glo 2.0 Cell Viability kit (Promega, G9242) was used to measure intracellular ATP (according to manufacturer instructions). Briefly, cells were plated in a 96-well plate at 8000 cells/well and treated accordingly. After 24 h, CellTiter-Glo reagent was added in an equal volume to promote cell lysis. ATP was quantified via luminescence measurement using Biotek Synergy plate reader.
3
Cytotoxicity Screening in Lung, Cardiac, and Vero Cells
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All compounds were tested in lung AT2, cardiac, and Vero cells in 96-well tissue culture plates. Vero cells were washed with 200 μL MEM. After removal of media, 100 μL cell-type-specific media containing diluted compound was added. At day 2 (kinase inhibitors) or day 3 (other compounds), cell viability was measuring using the CellTiter-Glo2.0 cell viability kit (Promega, Cat. G9241) as per the manufacturer’s recommendations. Luminescence was measured on FLUOstar Omega (BMG Labtech).
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