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Eosine

Manufactured by Merck Group
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Eosine is a laboratory dye used in various staining techniques for microscopy and histology. It is a red, water-soluble compound that binds to basic structures in cells, providing contrast and highlighting specific cellular components.

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Lab products found in correlation

Hematoxylin, by Merck Group (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Bovine catalase, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Formaldehyde, by Merck Group (1 mentions) Iodoacetic acid, by Merck Group (1 mentions) Nigrosine, by Merck Group (1 mentions) Rhodamine 123, by Merck Group (1 mentions) 3 n morpholino propanesulfonic acid, by Merck Group (1 mentions) Malondialdehyde, by Merck Group (1 mentions) Coomassie brilliant blue, by Merck Group (1 mentions) Oxidized glutathione, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions) Hydroxymethyl aminomethane hydrochloride tris hcl, by Merck Group (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Thiobarbituric acid, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Close spelling variants of this product

May-Grünwald’s eosine-methylene blue solution May-Grünwald Eosine-methylene blue solution Giemsa Azur-Eosine-methylene blue solution Hematoxyline-eosine Giemsa’s azur eosine methylene blue solution

7 protocols using eosine

1

Histochemical Analysis Protocol

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Acetic acid, NaCl, Hematoxylin, Eosine, Formaldehyde, Epeniphrine, Bovine catalase, Butulated hydroxytoluene (BHT), trichloroAcetic acid (TCA), 2-Thio-barbituric acid (TBA) NaOH and methanol, were from sigma chemicals Co (Germany). All other chemicals employed were of analytical grade.
Arrari F., Jabri M.A., Ayari A., Dakhli N., Ben Fayala C., Boubaker S, & Sebai H. (2024). Amino acid HPLC-FLD analysis of spirulina and its protective mechanism against the combination of obesity and colitis in wistar rats. Heliyon, 10(9), e30103.
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2

Visualizing β-Galactosidase Activity in Tissue

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For detection of β-galactosidase activity, cryosections from VEGFLacZ, VEGFR2+/LacZ or Nrp2+/LacZ mice were fixed in methanol and incubated (37°C) in X-gal [1 mg/ml X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) in DMSO; 5 mM K3Fe(CN)6; 5Mm K4Fe(CN)6; 2 mM MgCl2 in PBS; pH 6.5]. Sections were counterstained with eosin (E) (Sigma) and mounted with Permount.
Shahrabi-Farahani S., Wang L., Zwaans B.M., Santana J.M., Shimizu A., Takashima S., Kreuter M., Coultas L., D'Amore P.A., Arbeit J.M., Akslen L.A, & Bielenberg D.R. (2014). Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Laboratory investigation; a journal of technical methods and pathology, 94(7), 752-765.
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3

Sprague-Dawley Rat Inflammation Model

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A total of 25 male Sprague-Dawley rats (4 weeks old, weighing 80-100 g, Samtaco Bio) were used in the present study. The animals were housed in a controlled environment with a temperature of 24±1°C and a humidity of 50±5% with a 12 h light and dark cycle. Food and water were provided ad libitum. The rats (n=5 in each group) were intraperitoneally injected with CA (50 mg/kg). After 3 h, LPS (0.5 mg/kg) was inoculated subcutaneously into the right flanks of the rats. After 24 h, the rats were sacrificed, subcutaneous tissues were excised, and then fixed in 10% buffered formalin and embedded in paraffin. Tissue sections (4-µm-thick) were stained with Hematoxylin (Sigma-Aldrich; Merck KgaA) for 5 min and eosine (Sigma-Aldrich; Merck KgaA) for 10 sec at RT and then examination by light microscopy.
Kim N.Y., Trinh N.T., Ahn S.G, & Kim S.A. (2020). Cinnamaldehyde protects against oxidative stress and inhibits the TNF-α-induced inflammatory response in human umbilical vein endothelial cells. International Journal of Molecular Medicine, 46(1), 449-457.
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4

Evaluation of Oxidative Stress Markers

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2′,7′-Dichlorofluorescein diacetate (DCFH-DA), 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 4,2-Hydroxyethyl,1-piperazineethanesulfonic acid (HEPES), oxidized glutathione, dinitro fluoro benzene, bovine serum albumin, lithium chloride (Li+), 3-(N-morpholino) propane sulfonic acid (MOPS), ethylene glycol-bis (2-aminoethyl ether)-N, N, N′, N′-tetraacetic acid (EGTA), dimethyl sulfoxide, iodo-acetic acid, nigrosine, glutathione (GSH), malondialdehyde, potassium chloride, eosine, thiobarbituric acid, coomassie brilliant blue, sucrose, sodium chloride, dithiothreitol, rhodamine 123, and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Trichloroacetic acid (TCA) and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were purchased from Merck (Darmstadt, Germany).
Ommati M.M., Arabnezhad M.R., Farshad O., Jamshidzadeh A., Niknahad H., Retana-Marquez S., Jia Z., Nateghahmadi M.H., Mousavi K., Arazi A., Azmoon M.R., Azarpira N, & Heidari R. (2021). The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice. Frontiers in Veterinary Science, 8, 603262.
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5

Histological Analysis of Zebrafish Liver

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Liver samples taken from 1.5, 3, 4.5, 6, and 7.5 days post-DOX treatment (dpt) fish were slowly dehydrated using a series of increasing ethanol solution concentrations (70%, 90%, 95%, and 100%). Specimens were then embedded in paraffin using a Leica EG1120. Sectioning at 5 μm was performed using a Reichert-Jung 2030 microtome. Hematoxylin (H) (Vector Laboratories, Burlingame, CA, #H3404) and Eosin (E; Sigma, #HT110232) were used for H and E staining. Oil-Red-O (Sigma) staining of zebrafish liver sections was performed as previously described [18 (link)]. Identification and classification of tumor types were based on previously established criteria [6 (link)].
Monroe J.D., Fraher D., Huang X., Mellett N.A., Meikle P.J., Sinclair A.J., Lirette S.T., Maihle N.J., Gong Z, & Gibert Y. (2022). Identification of novel lipid biomarkers in xmrk- and Myc-induced models of hepatocellular carcinoma in zebrafish. Cancer & Metabolism, 10, 7.
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6

Visualizing β-Galactosidase Activity in Tissue

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For detection of β-galactosidase activity, cryosections from VEGFLacZ, VEGFR2+/LacZ or Nrp2+/LacZ mice were fixed in methanol and incubated (37°C) in X-gal [1 mg/ml X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) in DMSO; 5 mM K3Fe(CN)6; 5Mm K4Fe(CN)6; 2 mM MgCl2 in PBS; pH 6.5]. Sections were counterstained with eosin (E) (Sigma) and mounted with Permount.
Shahrabi-Farahani S., Wang L., Zwaans B.M., Santana J.M., Shimizu A., Takashima S., Kreuter M., Coultas L., D'Amore P.A., Arbeit J.M., Akslen L.A, & Bielenberg D.R. (2014). Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Laboratory investigation; a journal of technical methods and pathology, 94(7), 752-765.
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7

Virgin Coconut Oil Supplementation in SHR Rats

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SHR + coconut oil and SHR + trained + coconut oil groups were supplemented daily with virgin coconut oil in nature (2 mL•day -1 ). This dose was based on Nandakumaran et al. (2011) (link). Virgin coconut oil was administered by gavage and saline solution was used as vehicle. Dihydroethidium (DHE) was from Invitrogen (Carlsbad, Calif., USA). Hematoxiline and eosine were from Sigma-Aldrich (St. Louis, Mo., USA). Ketamine and xylazine were from Vetnil (Curitiba, PR, Brazil). Phenylephrine hydrochloride (PHE) and sodium nitroprusside (SNP) were from Sigma-Aldrich.
Alves N.F., Porpino S.K., Monteiro M.M., Gomes E.R, & Braga V.A. (2015). Coconut oil supplementation and physical exercise improves baroreflex sensitivity and oxidative stress in hypertensive rats. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 40(4).
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