From the above experiment, corneas were dissected after exposure, and sections were prepared for microscopy by setting cornea segments in OCT, quick freezing on dry ice and subsequent sectioning. Specimens of 7 μm thickness were prepared using a microtome (Bright, model 5040, UK) and cryostat (Bright, model OTF, UK) placed in groups of four to six on 75 mm × 25 mm glass slides, dried in a warm air stream for ∼10 min. Specimens were stained using Vectashield with DAPI mounting medium, and a glass coverslip applied. All cornea sections were examined within seven days of preparation using an AXIOCAM MRm 1.3 MP digital camera attached to a Zeiss AXIO Imager A1 fluorescent microscope, using AXIO Vs 40 V.4.8.2.0 software (Zeiss, Oberkochen, Germany). A 10× magnification eyepiece together with a 5× magnification objective lens was employed, and DAPI fluorescence filter was selected.
Axiocam mrm 1.3 mp digital camera
Manufactured by Zeiss
Sourced in Germany
The AXIOCAM MRm 1.3 MP digital camera is a high-quality imaging device designed for microscopy applications. It features a 1.3-megapixel sensor that captures detailed images. The camera is designed to be integrated with Zeiss microscopes and provides reliable performance for a variety of microscopy techniques.
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Lab products found in correlation
2 protocols using axiocam mrm 1.3 mp digital camera
1
Corneal Sectioning and Fluorescence Microscopy
2
Bovine Corneal Permeability Assay
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Experiments were carried out using fresh bovine eyes acquired from a local abattoir (P.C. Turners, Farnborough, UK). Whole eyes were collected from freshly slaughtered cows and transported in a cold box to the lab to be used immediately.
The testing method described by Mun et al (2014) was used. Each bovine eye was visually checked for corneal damage and placed in an 150 mL glass beaker. The donor part of a Franz cell was placed on top of the upward-facing cornea and fixed in place with cling film. The beaker was then placed in a 37°C water bath for 20 min, after which 1 mL of the samples was placed in the donor compartment for 1 h. The eye was taken out and washed with STF. The corneal area in contact with the sample plus 2 mm of surrounding corneal tissue was dissected. The cornea segments were immersed in OCT, frozen using dry ice and stored at -80°C until use.
Corneal cross-sectional specimens of 10 µm were prepared for microscopy using a microtome within a Bright cryostat (model OTF). They were stained using Vectashield mounting medium with DAPI. Slides were examined using an AXIOCAM MRm 1.3 MP digital camera mounted on a Zeiss AXIO Imager A1 fluorescent microscope with 50× magnification and a DAPI fluorescence filter was used. Photos were acquired using the AXIO Vision V4.8.2.0 software (Zeiss, Germany).
The testing method described by Mun et al (2014) was used. Each bovine eye was visually checked for corneal damage and placed in an 150 mL glass beaker. The donor part of a Franz cell was placed on top of the upward-facing cornea and fixed in place with cling film. The beaker was then placed in a 37°C water bath for 20 min, after which 1 mL of the samples was placed in the donor compartment for 1 h. The eye was taken out and washed with STF. The corneal area in contact with the sample plus 2 mm of surrounding corneal tissue was dissected. The cornea segments were immersed in OCT, frozen using dry ice and stored at -80°C until use.
Corneal cross-sectional specimens of 10 µm were prepared for microscopy using a microtome within a Bright cryostat (model OTF). They were stained using Vectashield mounting medium with DAPI. Slides were examined using an AXIOCAM MRm 1.3 MP digital camera mounted on a Zeiss AXIO Imager A1 fluorescent microscope with 50× magnification and a DAPI fluorescence filter was used. Photos were acquired using the AXIO Vision V4.8.2.0 software (Zeiss, Germany).
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