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Blocking buffer kit

Manufactured by Thermo Fisher Scientific
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The Blocking Buffer Kit is a laboratory reagent designed to prevent non-specific binding in immunoassay and molecular biology applications. The kit provides a ready-to-use blocking solution that can be used to block unoccupied binding sites on membranes, slides, or microplates, reducing background signal and improving the specificity of the assay.

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Lab products found in correlation

Alexa fluor 647 goat anti human igg a21445, by Thermo Fisher Scientific (2 mentions) Protoarray v5, by Thermo Fisher Scientific (1 mentions)

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Blocking buffer (88 citations)

2 protocols using blocking buffer kit

1

Autoantibody Screening Using ProtoArray Technology

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The autoantibody screening was performed by ISO9001 certified Custom ProtoArray Service Lab at Invitrogen (Thermo Fisher Scientific). Briefly, the protein arrays (ProtoArray v5.1) were probed as described in Invitrogen’s protocol for Immune Response BioMarker Profiling using detection reagent (Alexa Fluor 647 Goat Anti-Human IgG A21445, Invitrogen) and blocking buffer (Blocking Buffer Kit PA055, Invitrogen). Arrays were scanned using a GenePix 4000B fluorescent scanner, and the data were acquired with GenePix® Pro software. The arrays were probed with sera at a dilution of 1:500.
Fishman D., Kisand K., Hertel C., Rothe M., Remm A., Pihlap M., Adler P., Vilo J., Peet A., Meloni A., Podkrajsek K.T., Battelino T., Bruserud Ø., Wolff A.S., Husebye E.S., Kluger N., Krohn K., Ranki A., Peterson H., Hayday A, & Peterson P. (2017). Autoantibody Repertoire in APECED Patients Targets Two Distinct Subgroups of Proteins. Frontiers in Immunology, 8, 976.
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2

Probing Protein Arrays for Immune Response

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Probing and scanning of the protein arrays (ProtoArray® v5.0 PAH0525020, Life Technology) was conducted according to Invitrogen’s protocol for Immune Response BioMarker Profiling, using the recommended detection reagent (Alexa Fluor® 647 Goat Anti-Human IgG A21445, Invitrogen) and blocking buffer (Blocking Buffer Kit PA055, Invitrogen). The arrays were probed with sera at a dilution of 1:2000. Arrays were scanned using a GenePix 4000B microarray scanner, and the GenePix® Pro microarray (v6.1) software was used for alignment and data acquisition.
Landegren N., Sharon D., Freyhult E., Hallgren Å., Eriksson D., Edqvist P.H., Bensing S., Wahlberg J., Nelson L.M., Gustafsson J., Husebye E.S., Anderson M.S., Snyder M, & Kämpe O. (2016). Proteome-wide survey of the autoimmune target repertoire in autoimmune polyendocrine syndrome type 1. Scientific Reports, 6, 20104.
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