Fitc labeled goat anti rabbit igg antibody

MUSE-NPCs, 200 μL, at 1 × 10⁵/mL, were incubated in polylysine-coated 48-well plates. Each well contained 100 μL neural induction medium and 0, 30, 60, 90 or 120 ng/mL PDGF-BB. After 1, 3, 5 and 7 days of culture, cell growth was observed using the microscope. Cell proliferation rate was determined with the CCK-8 kit (Beyotime). Cells were cultured with 120 ng/mL PDGF-BB for 7 days, subjected to immunocytochemical staining using rabbit anti-MAP-2 antibody (1:1000; Millipore, CA, USA) and FITC-labeled goat anti-rabbit IgG antibody (1:500; Sigma-Aldrich), and observed by fluorescence microscopy (TCS SP8; Leica, Wetzlar, Germany).
MUSE-NPCs, 200 μL, at 1 × 10⁴/mL, were seeded in the upper chamber of a 6.5 mm Transwell (pore size: 8.0 µm; Corning, NY, USA). Neural induction medium, 500 μL, containing 0 or 120 ng/mL PDGF-BB, was added to the lower chamber. After 12 hours of culture, the membrane was stained with crystal violet. Four fields were randomly selected, and cells traversing to the lower chamber were quantified and analyzed with the light microscope (IX70; Olympus). For another experiment, 200 μL of a MUSE-NPC cell suspension, 1 × 10⁴/mL, was seeded into culture wells containing 10 mg of partition-type tubular scaffold and PDGF-MSs along with 120 ng/mL PDGF-BB and cultured for 1 month.