Frozen ALECSAT cells were thawed and washed in warm media prior to flow cytometry analysis. ALECSAT cells were stained with the following anti-human antibodies: CD3-PerCP-Cy5.5 (SK7), CD4-PE (SK3), CD8-APC (RPA-T8), CD16-APC-Cy7 (3G8) and CD56-PE-Cy7 (NCAM16.2) all from BD Biosciences. Samples were analyzed using a 4-laser BD LSRFortessa SORP (405, 488, 532 and 640 nm; BD Biosciences) and data was analyzed in FACSDiva Version 7 (BD Biosciences) or FlowJo version 10 (TreeStar).
Cd16 apc cy7 3g8
Manufactured by BD
Sourced in Canada
CD16-APC-Cy7 (3G8) is a fluorochrome-conjugated antibody that binds to the CD16 antigen, also known as the Fc gamma receptor III (FcγRIII). CD16 is expressed on natural killer cells, monocytes, macrophages, and a subset of T cells. The APC-Cy7 fluorochrome is used to label the antibody, allowing for detection and analysis using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable dead cell stain, by Thermo Fisher Scientific (2 mentions)
Streptomycin r5, by Wisent (2 mentions)
Cd57 pe tb01, by Thermo Fisher Scientific (2 mentions)
Nkg2c pe vio770 rea205, by Miltenyi Biotec (2 mentions)
Nkg2a apc rea110, by Miltenyi Biotec (2 mentions)
Lsrfortessa sorp, by BD (1 mentions)
Cd56 pe cy7 ncam16.2, by BD (1 mentions)
3 protocols using cd16 apc cy7 3g8
1
Multiparametric Analysis of ALECSAT Cells
2
Multicolor Flow Cytometry Analysis of Immune Cells
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Frozen PBMCs were thawed and resuspended in RPMI 1640; 5% fetal bovine serum; 2 mM L-glutamine; 50 international units/mL penicillin; 50 mg/mL streptomycin (R5) (all from Wisent, Inc., Saint-Jean-Baptiste, QC, Canada). LIVE/DEAD fixable dead cell stain (Invitrogen, Saint Laurent, QC, Canada) was added to the PBMCs as per manufacturer’s directions before surface staining cells using a panel that included the following fluorochrome conjugated antibodies to CD3-BV785 (clone OKT3), CD14-BV785 (M5E2), CD19-BV785 (HIB19), CD56-BV605 (HCD56) (all from BioLegend, San Diego, CA), CD57-PE (TB01) (Life Technologies, Burlington, ON, Canada), CD16-APC-Cy7 (3G8) (BD Bioscience, Baltimore, MD), NKG2C-PE-Vio770 (REA205) and NKG2A-APC (REA110) (Miltenyi Biotec, Auburn, CA).
3
Phenotyping of Human Immune Cells
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Frozen PBMCs were thawed and resuspended in RPMI 1640; 5% fetal bovine serum (FBS); 2 mM L-glutamine; 50 international units/mL penicillin; 50 µg/mL streptomycin (R5) (all from Wisent, Inc., Saint Jean-Baptiste, QC, Canada). LIVE/DEAD fixable dead cell stain (Invitrogen, Saint Laurent, QC, Canada) was added to the PBMCs as per the manufacturer’s directions before the cells were surface stained using a panel that included the following fluorochrome conjugated antibodies to CD3-BV785 (clone OKT3), CD14-BV785 (M5E2), CD19-BV785 (HIB19), CD56-BV605 (HCD56) (all from BioLegend, San Diego, CA, USA), CD57-PE (TB01) (Life Technologies, Burlington, ON, Canada), CD16-APC-Cy7 (3G8) (BD Bioscience, Baltimore, MD, USA), NKG2C-PE-Vio770 (REA205) and NKG2A-APC (REA110) (Miltenyi Biotec, Auburn, CA, USA) for 20 min at 4 °C.
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