Eclipse plus c18 5 μm column

¹H NMR were recorded on a Bruker Avance 400 MHz nuclear magnetic resonance spectrometer. All ¹H NMR spectra were calibrated to the NMR solvent reference peak (δ, d₆-DMSO, CDCl₃, CD₃OD) and are reported in parts per million (ppm). High-resolution mass spectrometry (HRMS) with electrospray ionization was performed by the Bioanalytical MS Facility at Portland State University. Inert atmosphere reactions were performed under argon gas passed through a small column of drierite and were conducted in flame-dried round-bottom flasks. Anhydrous tetrahydrofuran (THF), dichloromethane (DCM), and dimethylformamide (DMF) were obtained from a Seca Solvent System. All other solvents used were purchased from Sigma-Aldrich or Fisher. Purity analysis of final compounds was determined to be >95% by HPLC. HPLC analysis was performed on a Varian ProStar HPLC with an Agilent Eclipse Plus C18 5 μm column (4.6 × 250 mm) with a gradient of 10% to 95% acetonitrile (0.1% TFA) over 30 minutes.

¹H NMR were recorded on a Bruker Advance 400 MHz nuclear magnetic resonance spectrometer. All ¹H NMR spectra were calibrated to the NMR solvent reference peak (D₆-DMSO, CDCl₃, CD₃OD). High resolution mass spectrometry (HRMS) with electrospray ionization was performed by the Bioanalytical MS Facility at Portland State University. Inert atmosphere reactions were performed under argon gas passed through a small column of drierite and were conducted in flame-dried round-bottom flasks. Anhydrous tetrahydrofuran (THF), dichloromethane (DCM), and dimethylformamide (DMF) were obtained from a Seca Solvent System. All other solvents used were purchased from Sigma-Aldrich or Fisher. Purity analysis of final compounds was determined to be >95% by HPLC. HPLC analysis was performed on a Varian ProStar HPLC with an Agilent Eclipse Plus C18 5 μm column (4.6 × 250 mm) with a gradient of 10% to 95% acetonitrile (0.1% TFA) over 15 minutes.

¹H NMR were taken on a Bruker 400. All ¹H and ¹³C NMR were calibrated to the NMR solvent reference peak (D₆-acetone, CDCl₃). High resolution mass spectrometry (HRMS) with electrospray ionization was performed by the Bioanalytical MS Facility at Portland State University. Anhydrous tetrahydrofuran (THF) and dimethylformamide (DMF) were obtained from a Seca Solvent System. All other solvents used were purchased from Sigma-Aldrich or Fisher. Purity analysis of final compounds was determined to be >95% by HPLC. HPLC analysis was performed on a Varian ProStar HPLC with an Agilent Eclipse Plus C18 5 μM column (4.6 × 250 mm) with a gradient of 10% to 95% acetonitrile (0.1% TFA) over 15 minutes.