Mouse monoclonal anti myc

Manufactured by Thermo Fisher Scientific

Mouse monoclonal anti-myc is a laboratory reagent designed to detect the presence of the myc protein in various biological samples. It is a purified antibody produced in mice that specifically binds to the myc protein, allowing for its identification and quantification through various immunoassay techniques.

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Lab products found in correlation

Rabbit polyclonal anti lc3, by Cell Signaling Technology (1 mentions) Polyclonal rabbit anti β actin, by Cell Signaling Technology (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti gapdh, by Abcam (1 mentions)

Close spelling variants of this product

Anti-Myc (70 citations) Mouse anti-myc (12 citations) Mouse monoclonal anti-Myc antibody (7 citations) Mouse monoclonal anti c-Myc (clone 9E10) (2 citations)

2 protocols using mouse monoclonal anti myc

Immunoprecipitation and Western Blot Analysis

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A rabbit monoclonal anti-FLAG (1:50, Cell Signaling Technology, Danvers, MA) was used in co-immunoprecipitation experiments, whereas Western blots were probed with rabbit polyclonal anti-LC3 (Cell Signaling Technology), rabbit polyclonal anti-β actin (Cell Signaling Technology), rabbit polyclonal anti-p62 (Sigma), or mouse monoclonal anti-myc (Thermo Scientific, Waltham, MA) (all 1:1,000). Densitometry was undertaken with Image J software (Schneider et al., 2012 (link)).

Mahil S.K., Twelves S., Farkas K., Setta-Kaffetzi N., Burden A.D., Gach J.E., Irvine A.D., Képíró L., Mockenhaupt M., Oon H.H., Pinner J., Ranki A., Seyger M.M., Soler-Palacin P., Storan E.R., Tan E.S., Valeyrie-Allanore L., Young H.S., Trembath R.C., Choon S.E., Szell M., Bata-Csorgo Z., Smith C.H., Di Meglio P., Barker J.N, & Capon F. (2016). AP1S3 Mutations Cause Skin Autoinflammation by Disrupting Keratinocyte Autophagy and Up-Regulating IL-36 Production. The Journal of Investigative Dermatology, 136(11), 2251-2259.

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Immunodetection of Cellular Proteins

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The immunoreactivity of a desired protein was detected with a specific primary antibody that was purchased from a verified resource: mouse monoclonal anti-GAPDH (Abcam, cat# ab8245), mouse monoclonal anti-FLAG (Proteintech, cat# 66008-4-Ig), mouse monoclonal anti-Myc (ThermoFisher, cat # MA1-980), rabbit polyclonal antibody against p62 (Novus, cat# NBP1-48320), rabbit anti-DNAJB6 (Proteintech, cat# 11707-1-AP), rabbit anti-ACTG1 (Proteintech, cat# 11227-1-AP), and rabbit anti-PFN1 (ThermoFisher, cat# PA5-17444). Primary antibodies were diluted at 1:1,000 for immunoblotting and were used for immunostaining at the lowest dilutions recommended by the manufacturers.

Cui S., Zhang T., Xiong X., Zhao J., Cao Q., Zhou H, & Xia X.G. (2023). Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats. Frontiers in Neuroscience, 17, 1279259.

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