Ter119 ter 119

Manufactured by BioLegend

Ter119 (TER-119) is a monoclonal antibody that recognizes the Ter119 antigen, which is expressed on erythroid cells and their precursors. It is commonly used as a marker for the erythroid lineage in flow cytometry and other immunological applications.

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Lab products found in correlation

C kit 2b8, by BioLegend (2 mentions) Sca1 d7, by BioLegend (2 mentions) Cd34 ram34, by Thermo Fisher Scientific (2 mentions) B220 ra3 6b2, by BioLegend (2 mentions) Gr 1 rb6 8c5, by BioLegend (2 mentions) Cd45 30 f11, by BioLegend (2 mentions) Ki67 sola15, by Thermo Fisher Scientific (1 mentions) Foxp3 staining kit, by BD (1 mentions) Epcam g8.8, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Cd8a 53 6, by Thermo Fisher Scientific (1 mentions) Ly 6g c rb6 8c5, by BioLegend (1 mentions) Cd16 32 93, by Thermo Fisher Scientific (1 mentions) Cd105 mj7 18, by BioLegend (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Cd4 rm4 5, by BD (1 mentions) Cd11b m1 70, by BD (1 mentions) Cd150 tc15 12f12.2, by BioLegend (1 mentions)

Spelling variants of this product

Ter119 (116 citations) Anti-Ter119 (TER-119, (12 citations) Ter119-APC/Cy7 (clone TER-119; (7 citations) TER119 (clone TER-119) (4 citations) Anti-Ter119 (clone TER-119, (3 citations) TER119-biotin (TER-119, (2 citations) Biotin anti-TER119 (clone TER-119) (2 citations)

8 protocols using ter119 ter 119

Multiparametric Analysis of Immune Cells

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Mouse thymus, spleen, and lymph node (LN) were processed into single cell suspensions, and analyzed by flow cytometry. Thymic epithelial cells (TECs) were isolated as previously described (34 (link)). Dead cells were stained by a fixable viability dye conjugated to eFluor780 (eBioscience). Fixation and intracellular/intranuclear staining were performed using the eBioscience Foxp3 staining kit or BD Fixation/Permeabilization Solution Kit. The following antibodies were purchased from BD biosciences: TCRβ (H57–597), TCRγδ (GL-3) and EpCAM (G8.8). The following antibodies were purchased from eBioscience: CD3 (145–2C11), CD4 (GK1.5), CD11c (N418), CD25 (PC61.5), CD45R (B220, RA3–6B2), MHCII (M5/114) and Ki67 (SolA15). The following antibodies were purchased from BioLegend: CD8 (53–6.7), CD11b (M1/70), CD44 (IM7), CD45 (30-F11), CD62L (MEL-14), CD122 (TM-β1), Gr-1 (RB6–8C5), Ly-51 (6C3), NK-1.1 (PK136) and TER-119 (TER-119). UEA-1 was purchased from Vector Laboratories. Data were collected using a BD LSR II flow cytometer and were analyzed using FlowJo V10 (Treestar Inc.). The TaqMan Gene Expression Assay (ID: Mm00502000_m1, Thermo Fisher Scientific) for quantification of Klotho gene expression.

Xing Y., Smith M.J., Goetz C.A., McElmurry R.T., Parker S.L., Min D., Hollander G.A., Weinberg K.I., Tolar J., Stefanski H.E, & Blazar B.R. (2018). Thymic epithelial cell support of thymopoiesis does not require Klotho. Journal of immunology (Baltimore, Md. : 1950), 201(11), 3320-3328.

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Multiparametric Flow Cytometry Analysis

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All flow cytometric analyses and sorting were performed on a BD FACS Aria II cell sorter. Antibodies used were CD4 (RM4-5, BD Biosciences), CD8a (53-6.7, eBioscience), B220 (RA3-6B2, Biolegend), CD11b (M1/70, BD Biosciences), Ly6G/C (RB6-8C5, Biolegend), Ter119 (TER-119, Biolegend), Sca1 (D7, Biolegend), MPL (AMM2, Immuno-Biological Laboratories), CD41 (MWReg30, BD Biosciences), CD150 (TC15-12F12.2, Biolegend), cKit (2B8, Biolegend), CD34 (RAM34, eBioscience), IL7R (A7R34, eBioscience), Flt3 (A2F10, eBioscience), CD16/32 (93, eBioscience) and CD105 (MJ7/18, Biolegend). Streptavidin (eBioscience) was used to resolve biotinylated antibodies and propidium iodide was added prior to analysis to identify live cells.

O’Neill A., Chin D., Tan D., Majeed A.B., Nakamura-Ishizu A, & Suda T. (2020). Thrombopoietin maintains cell numbers of hematopoietic stem and progenitor cells with megakaryopoietic potential. Haematologica, 106(7), 1883-1891.

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Splenic Follicular B Cell Isolation

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Splenic B cells were enriched by magnetic depletion, removing non-B cells using the following antibodies coupled to biotin: CD11b (M1/70), CD11c (N418), CD4 (GK1.5), CD5 (53-7.3), CD8α (53-6.7), Gr1 (RB6-8C5), NK1.1 (PK136) as well as Ter119 (TER-119) (Biolegend), and the MagniSort Streptavidin Negative Selection Beads according to the manufacturer’s instructions (ThermoFisher). Alternatively, splenic CD19⁺B220⁺CD93^–IgM⁺CD1d⁺ Fo B cells were FACS-isolated on a FACSAriaIII (BD Biosciences).

Hagen M., Chakraborty T., Olson W.J., Heitz M., Hermann-Kleiter N., Kimpel J., Jenewein B., Pertoll J., Labi V., Rajewsky K, & Derudder E. (2022). miR-142 favors naïve B cell residence in peripheral lymph nodes. Frontiers in Immunology, 13, 847415.

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Multi-Parameter Cell Sorting and Analysis

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For cell sorting and analysis, monoclonal antibodies to CD41 (MWReg30, eBioscience), CXCR4 (2B11, eBioscience), CD11b (M1/70, eBioscience), F4/80 (BM8, eBioscience), Gr-1 (RB6-8C5, Biolegend), Ly6C (HK1.4, Biolegend), CD11c (N418, eBioscience), CD45.1 (A20, eBioscience), CD45.2 (104, Biolegend), CD4 (GK1.5, eBioscience), CD8 (53–6.7, Biolegend), INF-γ (XMG1.2, Biolegend), IL4 (11B11, Biolegend), CD34 (RAM34, eBioscience), Sca-1 (D7, Biolegend), c-kit (2B8, Biolegend), CD135 (A2F10, Biolegend), CD3ε (145–2 C11, Biolegend), CD45R (RA3-6B2, Biolegend), TER-119 (Ter-119, Biolegend), IgM (II/41, eBioscience), FγRII (93, Biolegend), IL-7R (A7R34, Biolegend), TNFα (MP6-XT22, Invitrogen), IL-6 (MP5-20F3, Biolegend), OVA257-264 (SIINFEKL) peptide bound to H-2K^b (eBio25-D1.16 (25-D1.16), Invitrogen) and IL-2 (JES6-5H4, eBioscience) were used where indicated.

Wang J., Xie J., Wang D., Han X., Chen M., Shi G., Jiang L, & Zhao M. (2022). CXCR4high megakaryocytes regulate host-defense immunity against bacterial pathogens. eLife, 11, e78662.

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Immune Cell Phenotyping Antibodies

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The following antibodies were used: CD90.2 (Thy 1.2) (53–2.1), CD45.1 (A20), LY6C (HK1.4), LY6G(1A8), CD71 (RI7217), Ter119 (TER-119) (BioLegend).

Sharma A., Mistriel-Zerbib S., Najar R.A., Engal E., Bentata M., Taqatqa N., Dahan S., Cohen K., Jaffe-Herman S., Geminder O., Baker M., Nevo Y., Plaschkes I., Kay G., Drier Y., Berger M, & Salton M. (2023). Isoforms of the TAL1 transcription factor have different roles in hematopoiesis and cell growth. PLOS Biology, 21(6), e3002175.

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Multiparametric Immunofluorescence Analysis of Murine Tissues

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The following primary antibodies were used for FACS and IF staining with murine tissues: Ter-119 (TER-119, Biolegend), CD45 (30-F11, Biolegend), EpCAM (G8.8, Santa Cruz Biotechnology), Pdpn (8.1.1, Biolegend), CD31 (390, Biolegend), Procr (eBio1560, Invitrogen), CD90 (53–2.1, Biolegend), CD55 (RIKO-3, Biolegend), αSMA (1A4, Sigma Aldrich), Adamdec1 (Origine #TA323936), Pcolce2 (Proteintech #10607-I-AP), C3 (11H9, Abcam), Sox6 (Abcam #ab30455), ER-TR7 (Abcam #ab51824), Collagen I (Abcam #ab34710), Collagen VI (Abcam #ab6588), and Fibronectin (Abcam #ab2413). The following secondary antibodies were used: donkey anti-rabbit PE (Poly4064, Biolegend), Alexa Fluor 488-, 546-, 568-, and 647-conjugated secondary antibodies were obtained for goat anti-rabbit, goat anti-rat, goat anti-mouse, and goat anti-Syrian hamster from Life Technologies, and DyLight 488- and 649-conjugated secondary antibodies for goat anti-Syrian hamster were obtained from Biolegend. NucBlue viability dye (Invitrogen) was spiked into single-cell suspensions before flow analysis or FACS sorting.
The following probes from Advanced Cell Diagnostics were used for FISH in murine tissues: Pi16 (Mm-Pi16-C2), Grem1 (Mm-Grem1-C3), and Agt (Mm-Agt-C1).

Jasso G.J., Jaiswal A., Varma M., Laszewski T., Grauel A., Omar A., Silva N., Dranoff G., Porter J.A., Mansfield K., Cremasco V., Regev A., Xavier R.J, & Graham D.B. (2022). Colon stroma mediates an inflammation-driven fibroblastic response controlling matrix remodeling and healing. PLoS Biology, 20(1), e3001532.

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Purification and Stimulation of Murine NK Cells

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NK cells were purified from spleen of wild type male mice by negative selection using magnetic beads and a cocktail of biotinylated antibodies: CD3e (145-2C11; BioLegend, 100304), CD4 (GK1.5; BioLegend,100404), CD8a (53-6.7; BioLegend, 100704), CD11b (M1/70; BioLegend, 101204), CD11c (N418; BioLegend, 117304), Gr-1 (RB6-8C5; BioLegend, 108404), TER-119 (TER-119; BioLegend, 1162204), B220 (RA3-6B2; BioLegend, 103204) and CD19 (6D5; BioLegend, 115504). Purified NK cells were plated at a density of 250,000 cells/mL in 2 mL of complete RPMI-1640 without FBS containing 25 ng/mL of rIL-27. 12 h later cells were lysed and used for gene expression analysis.

Aghayev T., Peshkova I.O., Mazitova A.M., Titerina E.K., Fatkhullina A.R., Campbell K.S., Grivennikov S.I., & Koltsova E.K. (2020). IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinoma.

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Isolation and Analysis of Bone Marrow Stromal Cells

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Tibias from Apoe -/-mice sacrificed at ZT1 or ZT13 were cleaned and crushed using a sterile scissor. After washing with 1X HBBS supplemented with 2% BSA and 10mM HEPES, fragmented bones were digested in DMEM + 0.2% collagenase D for 1 hour at 37 C. Digested tissue was washed twice and filtered through a 70 mm cell strainer. After centrifugation and red blood cell lysis, bone marrow cells were stained with CD45 (30-F11, BioLegend), TER-119 (TER-119, BioLegend), PDGF receptor b (APB5, Bio-Legend) and CD31 (MEC13.3, BioLegend). Bone marrow stromal cells were identified as CD45 -TER119 -CD31 -PDGF receptor b + cells. For CCL2 staining, cells were fixed and permeabilized using Fix/Perm Buffer (Biolegend) following manufacturer's instructions and stained using CCL2 (eH5, Thermo Fisher). Data were analyzed with FlowJo Software (10.1 Flowjo LLC). To assess expression levels of interest, geometrical mean fluorescence intensity (MFI) was analyzed.

Winter C., Silvestre-Roig C., Ortega-Gomez A., Lemnitzer P., Poelman H., Schumski A., Winter J., Drechsler M., de Jong R., Immler R., Sperandio M., Hristov M., Zeller T., Nicolaes G.A.F., Weber C., Viola J.R., Hidalgo A., Scheiermann C, & Soehnlein O. (2018). Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis. Cell metabolism, 28(1).

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