Briefly, 4 μm tissue slices were prepared from cryopreserved tissues and mounted on glass slides. Immunohistochemistry was performed according to the manufacturer’s instructions using an automated staining facility (Bond Max, Leica, Wetzlar, Germany). The following mouse monoclonal antibodies were utilized: CD3ϵ (clone ab16669, 1:100, Abcam, Cambridge, UK; RRID: AB_443425), CD4 (clone 4B12, 1:150, Leica, Wetzlar, Germany; RRID: AB_563559), CD8 (clone 4B11, 1:100, Leica, Wetzlar, Germany; RRID: AB_442068), CD20 (clone L26, 1:100, Leica, Wetzlar, Germany; RRID: AB_442055), CD163 (clone EPR19518, 1:500, Abcam, Cambridge, UK; RRID: AB_2753196), NKp46 (clone 195314, 1:175, R&D Systems, Minneapolis, MN, USA; RRID: AB_2149153) and FoxP3 (clone 236A/E7, 1:100, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_467555).
Cd163 clone epr19518
Manufactured by Abcam
Sourced in Germany, United Kingdom
CD163 (clone EPR19518) is a recombinant rabbit monoclonal antibody that recognizes the CD163 antigen. CD163 is a scavenger receptor expressed on the surface of monocytes and macrophages. The core function of this product is to detect and quantify CD163 expression.
Automatically generated - may contain errors
Lab products found in correlation
Bond max, by Leica (2 mentions)
Cd4 clone 4b12, by Leica (2 mentions)
Cd8 clone 4b11, by Leica (2 mentions)
Cd20 clone l26, by Leica (2 mentions)
Foxp3 clone 236a e7, by Thermo Fisher Scientific (2 mentions)
Cd3 clone ab16669, by Abcam (2 mentions)
Nkp46 clone 195314, by R&D Systems (2 mentions)
Ki 67 clone mib 1, by Agilent Technologies (1 mentions)
2 protocols using cd163 clone epr19518
1
Automated Immunohistochemistry Staining Protocol
2
Immunoprofiling of Pancreatic Ductal Adenocarcinoma
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The following mouse monoclonal antibodies were used to stain serial sections (4 µm) of cryopreserved PDA tissues and/or FFPE 3D bioprints: CD3ϵ (clone ab16669, 1:100, Abcam, UK; RRID: AB_443425), CD4 (clone 4B12, 1:150, Leica, Germany; RRID: AB_563559), CD8 (clone 4B11, 1:100, Leica, Germany; RRID: AB_442068), CD20 (clone L26, 1:100,Leica, Germany; RRID: AB_442055), CD163 (clone EPR19518, 1:500, Abcam, UK; RRID: AB_2753196), NKp46 (clone 195314, 1:175, R&D Systems, USA; RRID: AB_2149153), FoxP3 (clone 236A/E7, 1:100, Thermo Fisher Scientific, Germany; RRID: AB_467555), IL9 (clone EPR23484-151, 1:100, Abcam, UK), IL18 (clone EPR19954-188, 1:100, Abcam, UK), Granzyme B (clone 23H8L20, 1:200, Thermo Fisher Scientific, USA), Ki67 (clone MIB-1, 1:200, Dako, USA), LCK (clone 3A5, 1:50, Santa Cruz Biotechnology, USA). The complete staining procedure was carried out on a fully automated staining system (Bond-Max, Leica, Germany).
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