Ectopic stromal cells were seeded in 96-well plates (2 × 104 cells/well) and cultured at 37 °C with 5% CO2. 24 h later, ectopic stromal cells were incubated with different concentrations of GOx and BSA-GOx-NPs for another 12 h. 10 μL Cell Counting Kit-8 (E-CK-A362, Elabscience) was added to each well and incubated at 37 °C for 30 min. Then, the absorbance of plate was measured at 450 nm using a microplate reader (Nano Quant, Tecan).
E ck a362
Manufactured by Elabscience
Sourced in China
E-CK-A362 is a laboratory centrifuge designed for separating and concentrating samples. It can accommodate tubes or bottles of various sizes and has adjustable speed settings to accommodate different sample types and volumes.
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Lab products found in correlation
3 protocols using e ck a362
1
Cytotoxicity Assay of Ectopic Stromal Cells
2
Cell Proliferation Assay using CCK-8
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Cell counting kit-8 (CCK-8) (E-CK-A362, Elabscience Biotechnology) was utilized to evaluate the proliferative capacity of cells. Cells were seeded into a 96-well plate, then 10 μL CCK-8 reagent was introduced into each well at different timepoint of 24 hours, 48 hours, 72 hours and incubated for 2 hours at 37°C. Following this, the absorbance at 450 nm was determined. The average OD values of three wells were calculated, and three repeated experiments were performed.
3
Cytotoxicity Assay of PM2.5 Exposure
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After cells were cultured with PM2.5 for 24 hr, 10 μL/well CCK-8 solution (E-CK-A362, Elabscience Biotechnology Co.,Ltd, China) was added and incubated for 2 h. Then, the absorbance was measured at 450 nm using a microplate reader (Norgen Biotek Corp., Canada).
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