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Gr antibodies

Manufactured by Santa Cruz Biotechnology
Sourced in United States

GR antibodies are a type of laboratory equipment used for the detection and analysis of glucocorticoid receptors (GR) in biological samples. These antibodies specifically bind to GR proteins, allowing researchers to study their expression and distribution in various cells and tissues.

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2 protocols using gr antibodies

1

Immunohistochemical Analysis of Neuroinflammation

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MPTP, tamoxifen, MPP+, normal goat serum (NGS), diaminobenzidine, H2O2, carbenoxolone and ethidium bromide were purchased from Sigma-Aldrich. Vectastain Avidin-biotin peroxidase ABC kit, Vectashield and Vectamount mounting media were purchased from Vector. RNeasy Lipid Tissue Mini Kit was purchased from Qiagen. Superscript III reverse transcriptase kit and mouse ELISA TNF-α kit were purchased from InVitrogen. Iba-1 antibody was obtained from Wako. S100-beta and GFAP antibodies were purchased from Sigma-Aldrich; tyrosine hydroxylase (TH) and NeuN antibodies from Merck Millipore; GR antibodies from Santa Cruz (M20) and AbCam. Secondary biotinylated antibodies were purchased from Vector, whereas fluorescent secondary antibodies, anti-mouse Alexa 488, anti-rabbit Cy3 and anti-rabbit Alexa 633 were purchased from InVitrogen. Cx43-mimetic peptide Gap26 and  TAT-Gap19 were obtained from Pepnome Inc. and synthesized at >95% purity.
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2

Immunohistochemical Analysis of Glucocorticoid Receptor

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The lung tissues were fixed, sectioned, stained with hematoxylin (Thermo Shandon, PA, USA), and subjected to immunohistochemical staining with glucocorticoid receptor (GR) antibodies (Santa Cruz Biotechnology, USA). For quantitation, the optical densities of the GR protein were analyzed using Image-Pro Plus 6.0 software.
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