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Isotype matched control igg

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Isotype-matched control IgG is a laboratory reagent used to establish baseline binding levels in various immunoassays. It serves as a negative control by providing an antibody that does not bind to the target antigen of interest.

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Lab products found in correlation

Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions) Anti human cd38 percp cy5, by BD (1 mentions) Anti human cd19 pecy7, by BD (1 mentions) Anti human cd27 pe, by BD (1 mentions) Anti human cxcr5 percp cy5, by BD (1 mentions) Anti human cd3 bv510, by BD (1 mentions) Ficoll paque plus, by Cytiva (1 mentions) Facsaria 2, by BD (1 mentions) Pe conjugated anti cd36 antibody, by BD (1 mentions) Cd8 apc cy7, by BD (1 mentions) Cd4 bv605, by BD (1 mentions) Facs foterassa, by BD (1 mentions) Cd25 pe cy7, by BD (1 mentions)

Spelling variants of this product

Isotype controls (87 citations) IgG isotype control (34 citations) Control IgG (13 citations) Matched isotype control (10 citations) Isotype IgG (5 citations) Isotypes (2 citations)

4 protocols using isotype matched control igg

1

Integrin Blocking Protocol for Cell Function

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Hamster anti-β1-integrin (BD Biosciences,), isotype-matched
control IgM (BD Biosciences,), rat anti-α6-integrin (BD Biosciences,
555734) and isotype-matched control IgG (BD Biosciences, 553992) were used
at 100 μg/mL each for function blocking or control after overnight
dialysis into 1 liter DMEM/F-12 medium supplemented with 1× PenStrep
at 4°C with gentle agitation, using a dialysis cassette (Thermo
Fisher, 66383).
Wang S., Matsumoto K., Lish S.R., Cartagena-Rivera A.X, & Yamada K.M. (2021). Budding Epithelial Morphogenesis Driven by Cell-Matrix versus Cell-Cell Adhesion. Cell, 184(14), 3702-3716.e30.
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2

Phenotyping of Peripheral Blood Lymphocytes

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Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation using Ficoll-Paque Plus (Amersham Biosciences, Buckinghamshire, UK). The cells were then resuspended to 1 × 106 per mL in RPMI-1640 culture medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal calf serum, and stained with the following monoclonal antibodies: antihuman CD3-BV510, antihuman CD4-APC-H7, antihuman CXCR5-PerCP-Cy5.5, antihuman CXCR3-PE-Cy7, antihuman CCR6-PE, antihuman CD45RA-PE-CF594, antihuman ICOS-BV421, antihuman PD1-FITC, antihuman CD27-PE, antihuman CD20-APC-H7, antihuman CD38-PerCP-Cy5.5, antihuman CD19-PE-cy7 or isotype-matched control IgG (BD PharMingen San Diego, CA, U.S.). After washing twice with phosphate-buffered saline (containing 0.1% (w/v) NaN3), the samples were analyzed using FACS Aria II (BD Sciences, San Jose, USA); at least 50,000 events per sample were analyzed using FlowJo software (v7.6.2). The absolute count of cells was calculated on the basis of the cell proportion multiplied by the absolute number of lymphocytes.
Che Y., Qiu J., Jin T., Yin F., Li M, & Jiang Y. (2016). Circulating memory T follicular helper subsets, Tfh2 and Tfh17, participate in the pathogenesis of Guillain-Barré syndrome. Scientific Reports, 6, 20963.
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3

Multicolor Flow Cytometry Analysis

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PE-cy™5-conjugated mouse anti-CD41a antibody (clone HIP8), fluorescein isothiocyanate (FITC)-conjugated PAC-1, PE-conjugated anti-CD36 antibody and isotype-matched control IgG were from BD Biosciences/Pharmingen (San Jose, CA, USA).
Wang H., Yan W.H., Gong L., Song N.P., Wang C.X, & Zhong L. (2022). Platelet CD36 links overweight and a prothrombotic phenotype in patients with non-valvular atrial fibrillation. Frontiers in Cardiovascular Medicine, 9, 1066228.
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4

Multiparameter Flow Cytometry Profiling

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PBMCs were stained with different antibodies according to the manufacturer's instruction, in which antibodies used for the experiments included APC/ CY7-CD8, BV605-CD4, PE/CY7-CD25, FITC-PD-1, PE-Tim-3 or isotype-matched control IgG. All the antibodies and isotype controls were purchased from BD PharMingen San Diego, CA, USA. After staining, cells were washed twice with PBS and were subjected to flow cytometry analysis using a FACS Foterassa (BD, San Diego, CA, USA). Analyses of flow cytometry were performed by FlowJo software.
Liu Y., Yue L., Song X., Gu C., Shi X, & Wang Y. (2022). Dysfunction of peripheral regulatory T cells predicts lung injury after cardiopulmonary bypass. Bioscience trends, 15(6).
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