All fixed rat calvaria samples were decalcified with RapidCal immune (BBC Biochemical) solution with gentle shaking at 4 °C. The solution was changed every 2 days. After 21 days, the samples were dehydrated and embedded into paraffin. Next, 10 µm thick coronal sections were executed. The sectioned slides were stained with Hematoxylin & Eosin (Abcam, UK), Masson's Trichrome staining kit (VitroVivo Biotech), and immunohistochemistry staining (OCN, sc‐365797, Santacruz, TX, USA, 1:100). TRAP staining was performed with paraffin slides using TRAP staining kit (PMC‐AK04‐COS, Cosmo bio, Japan) following the provided protocol.
Rapidcal immune
Manufactured by StatLab
RapidCal immune is a laboratory instrument designed for the rapid and accurate measurement of calcium levels in various biological samples. The device utilizes a proprietary detection method to provide fast and reliable calcium analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rapidcal immune
1
Evaluating Bone Remodeling in Rat Calvaria
2
Paraffin Embedding and Histological Staining
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All fixed samples were incubated with RapidCal immune (BBC Biochemical) solution in gently shaking condition at room temperature. The solution was changed every 3 days. After decalcification, the samples were embedded into paraffin and sectioned at 10 μm. The sectioned slides were stained with H&E (Abcam) and MT (VitroVivo Biotech).
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