For immunohistrochemistry, formalin fixed tissue sections were stained for human mitochondria using primary Human Mitochondria monoclonal antibody MAB1273 (Millipore, Billerica, MA). All the H&E and IHC stained slides were scanned by using the Aperio ScanScope imaging platform (Aperio Technologies, Vista, CA) with a 20x objective at a spatial sampling period of 0.47 μm per pixel. Whole slides images (WSI) were viewed and analyzed by using desktop personal computers equipped with the free ScanScope software. For immunofluorescence, formalin fixed tissues were stained using Alexa Fluor 488 conjugated anti-mitochondrial antibody MAB1273A4 (Millipore, Billerica, MA), counterstained and mounted in ProLong Gold Antifade mounting media (Thermo Fisher). Immunofluorescence images were acquired on a Zeiss LSM880 confocal microscope.
Mab1273a4
Manufactured by Merck Group
MAB1273A4 is a laboratory equipment product. It is designed to perform specific laboratory functions. The core function of this product is to facilitate laboratory operations.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 880 confocal microscope, by Zeiss (2 mentions)
Mab1273, by Merck Group (1 mentions)
Prolong gold antifade mounting media, by Thermo Fisher Scientific (1 mentions)
Aperio scanscope, by Leica (1 mentions)
Optiview universal dab detection kit, by Roche (1 mentions)
Nkcc2, by StressMarq Biosciences (1 mentions)
Red 610 kit, by Roche (1 mentions)
Pdgfra, by Roche (1 mentions)
Col1a1, by Novus Biologicals (1 mentions)
Pdgfra, by R&D Systems (1 mentions)
Hematoxylin and eosin, by Roche (1 mentions)
Benchmark ultra, by Roche (1 mentions)
2 protocols using mab1273a4
1
Visualizing Mitochondrial Protein Expression
2
Immunohistochemical Profiling of Organoid Structures
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Four-micron sections of formalin-fixed paraffin embedded organoids were stained with hematoxylin and eosin (Ventana Medical Systems, Tucson, AZ). Immunohistochemistry was performed with an automated, validated, and accredited staining system (Ventana Benchmark ULTRA) using the UltraView or OptiView universal DAB detection Kit (Ventana Medical Systems). After deparaffinization and heat-induced antigen retrieval, the tissue samples were incubated with WT1 (Cell Marque, Rocklin, CA), ECAD (Ventana Medical Systems), Villin-1 (Abcam, Cambridge, UK), CD31 (Cell Marque), renin (Abcam), NKCC2 (StressMarq Biosciences, Victoria, BC, Canada), NHE3 (StressMarq), CD34 (Cell Marque), COL1A1 (Novus Biologicals, Littleton, CO), or PDGFRa (R&D systems). Incubation was followed by hematoxylin II counterstaining. Double stainings of PDGFRa and renin were performed by incubating the samples with PDGFRa for 32 minutes at 37 C followed by detection with Red610 kit (#760-245, Ventana) and renin for 32 minutes at 37 C followed by detection with FAM kit (#760-243, Ventana). An AF488-conjugated anti-human mitochondria antibody MAB1273A4 (Millipore, Billerica, MA) was used to detect
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