Immobilized metal affinity chromatography resin

Unless otherwise specified, all reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Luria-Bertani (LB) medium and isopropyl-β-D-thiogalactopyranoside (IPTG) were purchased from AMRESCO LLC (Cochran Road Solon, OH, USA). Glycerol-3-phosphate dehydrogenase (α-GDH) and reduced nicotinamide adenine dinucleotide (NADH) were purchased from Roche (Penzberg, Upper Bavaria, Germany). Immobilized Metal Affinity Chromatography (IMAC) resin was purchased from Bio-Rad (Hercules, California, USA). Amicon Ultra 30 kDa filters were purchased from Millipore Corporation (Billerica, Massachusetts, USA).
The sulfhydryl reagents utilized were methylmethane thiosulfonate (MMTS), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) and sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES). The last was purchased from Toronto Research Chemicals (Toronto, Canada).
The commercially approved drugs used were omeprazole, rabeprazole and sulbutiamine. The last was purchased from Santa Cruz, Biotechnology (Dallas, Texas, USA).

Luria–Bertani (LB) medium and isopropyl-β-d-thiogalactopyranoside (IPTG) were purchased from VWR Life Science Products (Radnor, Pennsylvania, USA). Glycerol-3-phosphate dehydrogenase (α-GDH), l-lactate dehydrogenase from rabbit muscle and reduced nicotinamide adenine dinucleotide (NADH) were obtained from Roche (Penzberg, Upper Bavaria, Germany). Immobilized metal affinity chromatography (IMAC) resin was obtained from Bio-Rad (Hercules, California, USA). Sephadex G-25 Fine Resin was obtained from Amersham Biosciences (Amersham, UK). Amicon Ultra 30 kDa filters were purchased from Merck-Millipore Corporation (Billerica, Massachusetts, USA). Fetal bovine serum (FBS), penicillin, streptomycin and trypsin EDTA solutions were purchased from Invitrogen (Carlsbad, USA). The other reagents mentioned were acquired from Sigma-Aldrich (St. Louis, MO, USA).