Anti fancd2 epr2302

Antibodies purchased from Sigma‐Aldrich, with dilutions for immunoblotting, were as follows: F3165, monoclonal anti‐FLAG 1:10,000; T5168, monoclonal anti‐α tubulin 1:8,000; A0168 HRP (horseradish peroxidase)‐conjugated anti‐mouse IgG 1:10,000, A0545 HRP‐conjugated anti‐rabbit IgG 1:10,000; HPA036582, polyclonal anti‐FAM35A 1:200. D153‐8 agarose‐conjugated anti‐GFP (RQ2) was purchased from MBL International Corporation. From Clontech, polyclonal anti‐GFP antibody 632592 was used at 1:200 (for immunostaining); monoclonal anti‐GFP 632381, 1:3,000. Anti‐RAD51 antibody (B01P, 1:2,000) was purchased from Abnova. Goat anti‐mouse IgG (H+L) secondary antibody, Alexa Fluor^® 594 conjugate (A‐11005) 1:2,000, goat anti‐rabbit IgG (H+L) secondary antibody, Alexa Fluor^® 488 conjugate (A‐11008) 1:2,000, and goat anti‐mouse IgG (H+L) secondary antibody, Alexa Fluor^® 488 conjugate (A‐11001) 1:2,000 were purchased from Thermo Fisher. Anti‐FANCD2 (EPR2302, 1:2,000 dilution) was purchased from GeneTex, Inc. From Cell Signaling Technology, monoclonal anti‐HA (C29F4) was used at 1:1,000.

Anti-FANCL (ab42639-100, 1:500 dilution) and anti-BRCA1 (ab16780, 1:1000 dilution) were purchased from Abcam. Anti-FANCA (A301-980A, 1:10,000 dilution) was purchased from Bethyl. Anti-FANCD2 (EPR2302, 1:2000 dilution) was purchased from GeneTex Inc. Anti-HELQ (1:1000 dilution) was from Cell Signaling Technology. Anti-α-tubulin (T5168, 1:8000 dilution), horseradish peroxidase (HRP)-conjugated anti-mouse IgG (A0168, 1:20,000 dilution), HRP conjugated anti-rabbit IgG (A0545, 1:20,000 dilution), anti-FLAG M2 (F3165), and anti-FLAG M2 affinity agarose gel (A2220) were purchased from Sigma. Anti-V5 antibody (R962-25) was purchased from Life Technologies, Inc. Anti-POLN (PA434) was raised against purified recombinant POLN (2 (link), 3 (link)).